中国动物传染病学报Issue(4)：35-39,5.
猪Mx1基因的克隆表达及抗病毒活性研究
EUKARYOTIC EXPRESSION AND ANTIVIRAL ACTIVITY OF PORCINE MYXOVIRUS RESISTANCE GENE (Mx1)
摘要
Abstract
The present study was to clone Myxovirus resistance protein Mx1 for eukaryotic expression and detect its antiviral activity. The RNA coding for Mx1 was extracted from PK15 cells that were stimulated for 7 hours with swine fever vaccine and Poly real:IC. Subsequently, full-length Mx1 gene was amplified in RT-PCR using primers designed to rescue 11 bp missing in PK 15 cell line. The recombinant expression plasmid pRetroQ-sMx1 was constructed and used to transfect HEK293T cells for expression. Success of Mx1 expression in HEK293T cells was confirmed through double enzyme identification, determination of nucleic acid sequence, detection of green fluorescence and Western blot. Cytopathic effect inhibition assay was used to measure antiviral activity of expressed Mx1.关键词
Mx1基因/克隆/真核表达/抗病毒活性Key words
Mx1 gene/clone/eukaryotic expression/antiviral activity分类
农业科技引用本文复制引用
刘玉洁,赵协,张廷虹,曹广明,扬志昆..猪Mx1基因的克隆表达及抗病毒活性研究[J].中国动物传染病学报,2014,(4):35-39,5.基金项目
山东省科技攻关项目 （）
