中国动物传染病学报Issue(4)：56-62,7.
2012~2013年山东省禽源大肠杆菌中质粒介导喹诺酮类药物耐药基因的检测
DETECTION OF PLASMID-MEDIATED QUINOLONE RESISTANCE GENES IN AVIAN ESCHERICHIACOLI ISOLATES FROM SHANDONG PROVINCE IN 2012-2013
摘要
Abstract
To investigate the distribution of plasmid-mediated quinolone resistance (PMQR) genes and their effect on the bacterial resistance to the quinolone antibiotics in recent years, qnrA, qnrB, qnrC, qnrD, qnrS, oqxA, oqxB and qepA genes of 93 avian E. coli strains isolated from Shandong Province were detected individually in PCR, and the resistance to Quinolones (Ciprofloxacin, Lomefloxacin, Ofloxacin, Norfloxacin, Pefloxacin) was detected in antimicrobial susceptibility test. Among 93 E.coli isolates tested, 47 (50.54%) to 80 (86.30%) strains were resistant to five Quinolones respectively, and 56 (60.21%) strains were positive to PMQR genes. In addition, 25 isolates (26.88%) harbored two kinds of PMQR genes and 1 isolates (1.07%) harbored three kinds of PMQR genes. The positive rates of qnrS, oqxA and oqxB gene were 22.58%(21/93), 40.86%(38/93) and 24.73%(23/93) , respectively. None of qnrA, qnrB, qnrC, qnrD and qepAgenes was detected.关键词
禽源致病性大肠杆菌/药敏试验/质粒介导喹诺酮类药物耐药基因/PCRKey words
Avian pathogenic Escherichiacoli/drug susceptibility test/PMQR genes/PCR分类
农业科技引用本文复制引用
孔令超,李志国,张瑞华,焦玉祥,马明杰,郭翠平,朱岩丽,谢之景,姜世金..2012~2013年山东省禽源大肠杆菌中质粒介导喹诺酮类药物耐药基因的检测[J].中国动物传染病学报,2014,(4):56-62,7.基金项目
山东省现代农业产业技术体系家禽创新团队 （）
