中国动物传染病学报Issue(5)：28-34,7.
猪圆环病毒2型Cap蛋白表达及免疫原性鉴定
EXPRESSION AND IMMUNOGENICITY OF RECOMBINANT CAP PROTEIN OF PORCINE CIRCOVIRUS TYPE 2
朱鹏 1张小飞 2徐延伟 3尹秀凤3
作者信息
- 1. 安徽农业大学动物科技学院，合肥230036
- 2. 南京天邦生物科技有限公司 生物技术研究所，南京211102
- 3. 南京天邦生物科技有限公司 生物技术研究所，南京211102
- 折叠
摘要
Abstract
The truncated ORF2 of Porcine circovirus type 2(PCV2) WH strain was amplified and cloned into expression vector pET32a (+). The expression vector pET32a-Cap2 was then transformed into BL2 (DE3) for expression with induction of IPTG. The expressed pET32a-Cap products were analyzed in SDS-PAGE and Western blot. The recombinant Cap protein was a soluble protein with molecular weight of 40 kDa. The purified Cap protein was used to immunize mice to prepare antiserum. The resulting murine antiserum had ELISA titer at over 1:25 600 and reacted with PCV2 in infected PK-15 cells in indirect immunofluorescence assay (IFA). The murine antiserum also showed neutralizing titer at 1:36. The availability of the recombinant PCV2 Cap protein provides the basis for development of vaccine and diagnostic reagents.关键词
猪圆环病毒2型/Cap蛋白/免疫原性/中和活性Key words
Porcine circovirus type 2/Cap protein/immunogenicity/neutralization分类
农业科技引用本文复制引用
朱鹏,张小飞,徐延伟,尹秀凤..猪圆环病毒2型Cap蛋白表达及免疫原性鉴定[J].中国动物传染病学报,2014,(5):28-34,7.
