中国动物传染病学报Issue(6)：6-12,7.
一株Nsp2蛋白部分氨基酸自然缺失的猪繁殖与呼吸综合征病毒的分离和鉴定
ISOLATION AND IDENTIFICATION OF A PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS FIELD STRAIN WITH NATURAL DELETION OF PARTIAL NSP2 PROTEIN
摘要
Abstract
The lung tissues with Porcine reproductive and respiratory syndrome virus(PRRSV) RT-PCR positive results were homogenized and inoculated into MARC-145 cells for virus isolation. PRRSV typical CPE was observed at the second passage. PRRSV RNA was detected in supernatants using RT-PCR. PRRSV N protein was confirmed in inoculate MARC-145 cells. The obtained PRRSV was designated as NN1396. Hypervariable region of GP5 protein was sequenced and analyzed. The GP5 of NN1396 strain shared 62.9%-98.2%identities with other PRRSV strains. Phylogenetic analysis showed that NN1396 strain belonged to North American genotype and HP-PRRSV. Sequence analysis of partial nsp2 gene showed that NN1396 strain had 81.4%amino acid identity with VR-2332 strain and 96.5%with JXA1. More importantly, NN1396 strain contained a deletion of 19 aa in nsp2 coding region , which was located upstream of 29 aa deletion. Characterization of PRRSV NN1396 strain was useful to study the effect of partial gene deletion of nsp2 on its biological activities.关键词
高致病性猪繁殖与呼吸综合征病毒/分离鉴定/GP5/Nsp2部分氨基酸自然缺失Key words
Highly pathogenic Porcine reproductive and respiratory syndrome virus(HP-PRRSV)/isolation/GP5/natural deletion of partial Nsp2 protein分类
农业科技引用本文复制引用
洪绍锋,韦莹,韦苗苗,覃陆英,覃艳然,陈樱,黄伟坚,韦祖樟..一株Nsp2蛋白部分氨基酸自然缺失的猪繁殖与呼吸综合征病毒的分离和鉴定[J].中国动物传染病学报,2014,(6):6-12,7.基金项目
广西自然科学基金（桂科自0728015）；国家自然科学基金（31372444）；广西大学大学生实验技能和科技创新能力训练基金项目 （）
