中国动物传染病学报Issue(6)：13-17,5.
古典H1N1亚型猪流感病毒反向遗传操作平台的建立
THE ESTABLISHMENT OF REVERSE GENETIC SYSTEM OF CLASSICAL H1N1 SWINE INFLUENZA VIRUS
摘要
Abstract
Eight gene segments of Influenza virus A/Swine/Guangdong/1/2011(H1N1) were amplified by RT-PCR, separately cloned into the vector of pBD and then transfected together into 293T cells. The supernatant of 293T cells was collected after 48 hours and inoculated into MDCK cells. When cytopathic effects(CPE) appeared in MDCK cells, virus titer was determined at 1:64 in hemagglutination test, which was comparable to the original wild strain. Eight genes of the rescued viruses were amplified in RT-PCR and also confirmed to be identical to the original virus. Therefore, rescued viruses were the same as the original influenza virus. The establishment of reverse genetic system of H1N1 subtype Swine influenza virus successfully, not only lay the foundation for exploring for the pathogenic mechanism, the infection mechanism and function, and so on, but also provide a basis for influenza vaccine of H1N1 swine influenza in the future.关键词
RT-PCR/H1N1亚型/猪流感病毒/反向遗传/病毒拯救Key words
RT-PCR/H1N1 subtype/Swine influenza virus/reverse genetics/virus rescue分类
农业科技引用本文复制引用
汪秀会,宫晓倩,刘晓敏,阮宝阳,李泽君,周艳君,童光志,于海..古典H1N1亚型猪流感病毒反向遗传操作平台的建立[J].中国动物传染病学报,2014,(6):13-17,5.基金项目
国家青年自然科学基金（31201916）；上海市自然科学基金青年项目（12ZR1453500）；中国农业科学院创新工程团队“动物流感病毒病原生态学” （）
