中国动物传染病学报Issue(6)：18-24,7.
肠炎沙门菌I型菌毛主要亚单位FimA原核表达及纯化
PROKARYOTIC EXPRESSION AND PURIFICATION OF MAJOR SUBUNIT FIMA OF TYPE I FIMBRIAE OF SALMONELLAENTERITIDIS
摘要
Abstract
To investigate if the non-coding small RNA IsrE of Salmonella enteritidis was involved in regulating FimA at the post-transcriptional level, fimA gene was cloned in pET-28a(+) vector that was then transformed into E.coli BL21(DE3). The expression conditions of FimA were optimized and expressed FimA was purified using Ni2+-chelating chromatography. The recombinant FimA was visualized as a single clear band of a relative molecular weight at 19.3 kDa in SDS-PAGE. The availability of highly purified recombinant FimA provided the foundation and platform for further study on whether or not FimA acted as a candidate target gene for small non-coding RNA IsrE of Salmonellaenteritidis at the post-transcriptional level.关键词
肠炎沙门菌/非编码sRNA/IsrE/FimA/原核表达Key words
Salmonellaenteritidis/non-coding small RNA/IsrE/FimA/prokaryotic expression分类
农业科技引用本文复制引用
王勇祥,孟霞,段进坤,周明旭,陶洁,杨溢,朱国强..肠炎沙门菌I型菌毛主要亚单位FimA原核表达及纯化[J].中国动物传染病学报,2014,(6):18-24,7.基金项目
国家自然科学基金（31101826，31270171，31101833）；国家科技支撑计划（2012BAK17B10）；教育部创新团队（IRT0978）；江苏高校优势学科建设工程资助项目(PAPD)；江苏省自然科学基金（BK2012265，BK2011430）；扬州市农业科技攻关计划项目 （PAPD）
