中国动物传染病学报Issue(6)：32-37,6.
巨型艾美耳球虫早熟株免疫剂量研究
STUDY ON THE IMMUNIZING DOSAGES OF A PRECOCIOUS LINE OF EIMERIAMAXIMA
摘要
Abstract
In order to determine optimal immunization dose of a precocious line of Eimeria maxima, the first trial was conducted including 7 groups immunized with a precocious line, 7 groups immunized with its parent strain, 1 unimmunized-challenged group and 1 unimmunized-unchallenged group. Dosages of 100, 200, 400, 600, 800, 1000 and 2000 sporulated oocysts of the precocious line or its parent strain were administered orally to 7-day-old chickens for twice at a 7-day interval. Seven days later, all chickens in the challenged grorps were challenged with 8×104 sporulated oocysts of the parent strain. Weight gains, intestinal lesion scores and reduction of oocyst ratios were used to evaluate the efficacy. Weight gains of immunization groups with 200-2000 sporulated oocysts of the precocious line and parent strain had no significant difference from those of unimmunized-unchallenged group (P>0.05) while weigh gains of immunization groups with 100 sporulated oocysts were significantly lower than those of unimmunized-unchallenged group (P<0.05). Oocyst production were reduced over 90%in all immunization groups. Intestinal lesion scores of all immunization groups were similar to those of unimmunized-challenged group (P>0.05). The effectiveness of doses of 200 and 400 sporulated oocysts of precocious line were repeated. Both weight gains and intestinal lesion scores of these two immunization groups were similar to those of unimmunized-unchallenged group (P>0.05), but significantly different from those of unimmunized-challenged group (P<0.05). Reduction of oocysts ratios was over 86%. Results from both trials demonstrated that the precocious line of E. maxima maintains good immunogenicity and could induce sufficient immunological protection at 200/400 immunizing dosage. Therefore, 200 sporulated oocysts were determined to be optimal immunization dose.关键词
球虫/巨型艾美耳球虫/早熟株/免疫Key words
Coccidia/Eimeriamaxima/precocious line/immunization分类
农业科技引用本文复制引用
赵其平,朱顺海,韩红玉,李莎,翟颀,梁思婷,杨斯涵,黄兵,董辉..巨型艾美耳球虫早熟株免疫剂量研究[J].中国动物传染病学报,2014,(6):32-37,6.基金项目
上海市科委重点科技攻关项目 （）
