中国动物传染病学报Issue(6)：46-52,7.
日本血吸虫四跨膜孤儿受体（TOR）克隆及其第一个膜外区基因的表达
CLONING OF TRISPANNING ORPHAN RECEPTOR OF SCHISTOSOMA JAPONICUM AND EXPRESSION OF ITS FIRST EXTRACELLUAR DOMAIN
摘要
Abstract
Trispanning orphan receptors of Schistosomahaematobium (ShTOR) and S.mansoni (SmTOR) can bind to complement C2a, which may play an important role in immune evasion of Schistosoma. To characterize functions of Schistosomajaponicum tetraspanning orphan receptor (SjTOR), entire SjTOR ORF was amplified in PCR and its sequence was analyzed in bioinformatics. Moreover, the N-terminal extracellular domain 1 (ed1) of the SjTOR gene was subcloned into vector pET-28a (+) to construct a recombinant plasmid pET-28a-SjTOR-ed1. The recombinant rSjTOR-ed1 was expressed in Escherichiacoli BL21 (DE3) and purified using a Ni-NTA His•Bind Resin. Western blot analysis showed that rSjTOR-ed1 reacted with schisosomasis-positive mouse serum. The results from the present study were useful to characterize functions of SjTOR in future study.关键词
日本血吸虫/四跨膜孤儿受体/生物信息学/基因克隆/Western blotKey words
Schistosomajaponicum/trispanning orphan receptor/bioinformatics/cloning/Western blot分类
农业科技引用本文复制引用
马帅,陆珂,贾秉光,林矫矫,傅志强,韩艳辉,洪炀,张旻,曹晓丹,韩倩,刘艳涛,陆看,马茜茜..日本血吸虫四跨膜孤儿受体（TOR）克隆及其第一个膜外区基因的表达[J].中国动物传染病学报,2014,(6):46-52,7.基金项目
国家自然科学基金资助项目(31472188） （）
上海市科技发展基金(12140902700) （12140902700）
