中国动物传染病学报Issue(1)：9-14,6.
犬源伪狂犬病毒的分离与鉴定
ISOLATION AND IDENTIFICATION OF PSEUDORABIES VIRUS FROM DOGS
摘要
Abstract
In the winter of 2012 and 2013, some canine cases were initially diagnosed as Pseudorabies virus(PRV) infection according to their clinical signs at a pet animal hospital in Changchun. The brain tissues of the diseased dogs were submitted to our laboratory for confirmation. The examination under electron microscope, animal experiment and PCR on the brain tissues confirmed positive results. Two virus strains were obtained and designated as JLCC-2012 and JLCC-2013. Their gC gene fragments were amplified using respective primers. Sequence analysis of gC gene showed 100%homology between JLCC-2012 and JLCC-2013. Further phylogenetic analysis of nucleotide sequence of gC gene revealed that these two strains had identity at 99.93%and 100%with recently isolated canine BJ/RD and BJ/YT strains, 94.33%-100%with swine PRV strains kept in our laboratory and 93.58%-94.81%with foreign swine PRV strains published in the literature. The results confirmed that those canine cases were infected with PRV.关键词
伪狂犬病毒/分离/鉴定/感染/基因分析/犬Key words
Pseudorabies virus/isolation/identification/infection/phylogenetic analysis/dogs分类
农业科技引用本文复制引用
靳红亮,刘晔,李楠,李志君,赵敬慧,王述超,张守峰,扈荣良..犬源伪狂犬病毒的分离与鉴定[J].中国动物传染病学报,2015,(1):9-14,6.基金项目
863计划 （）
