中国动物传染病学报Issue(1)：39-46,8.
日本血吸虫SjCHGC06822蛋白诱导BALB/c小鼠免疫保护效果观察
IMMUNE PROTECTIVE RESPONSE OF RECOMBINANT SJCHGC06822 PROTEIN AGAINST SCHISTOSOMAJAPONICUM
摘要
Abstract
The present study aimed at obtaining the recombinant SjCHGC06822 protein by using prokaryotic expression system and evaluating the immune response in BALB/c mice. A full-length ORF encoding SjCHGC06822 of S.japonicum was amplified in PCR. The obtained cDNA was subcloned into pET28a(+) vector that was then transformed into BL21(2E). The recombinant protein was purified using His-Ni resin. Bioinformatics analysis showed that the full-length ORF was 591 bp, encoding 192 amino acids. The SjCHGC06822 protein had an EF-hand domain without signal peptide and transmembrane peptide. The recombinant protein was visualized as a band at molecular mass of 23 kDa in Western blot. BALB/c mice were immunized with the recombinant protein and antibody responses were detected in ELISA. The immunized mice along with non-immunized controls were challenged with S.japonicum and worm reduction rate and liver egg reduction were recorded. In two separate animal experiments, worm reduction rates of the immunized mice were 30.36%and 40.26%and liver egg reductions were 30.14%and 12.28%. The specific IgG, IgG2a and IgG1 were detected using ELISA. The antibody responses gradually increased post immunization and subsequent challenge. However, IgG2a and IgG1 levels increased post immunization but decreased post challenge while the ratio of IgG2a/IgG1 (<1) was climbing. In conclusion, the recombinant SjCHGC06822 protein can induce good immunogenicity.关键词
日本血吸虫/SjCHGC06822/免疫原性/Th1应答Key words
Schistosomajaponicum/SjCHGC06822/immunogenicity/Th1 type response分类
农业科技引用本文复制引用
段明明,刘群,葛程,苑纯秀,陆柯,李浩,冯新港..日本血吸虫SjCHGC06822蛋白诱导BALB/c小鼠免疫保护效果观察[J].中国动物传染病学报,2015,(1):39-46,8.基金项目
中央级公益性科研院所基本科研业务费项目 （）
