中国动物传染病学报Issue(1)：54-59,6.
四川省山羊多头蚴线粒体cox2基因序列测定及种系发育分析
SEQUENCING AND PHYLOGENETIC ANALYSIS OF MITOCHONDRIAL COX2 GENE OF COENURUS IN GOATS IN SICHUAN PROVINCE
摘要
Abstract
The mitochondrial cytochrome coxidase subunit 2 (cox2) gene was used as a molecular marker to identify the intraspecies variation and reconstruct their phylogenetic relationship of 12 Coenurus isolates collected from goats in Sichuan province. Sequence analysis indicated that the cox2 gene fragment was 531 bp containing a total of 8 nucleotide variant sites. The Phylogenetic tree based on the cox2 sequence showed that all Taeina multiceps isolates clustered together while cerebral and intramuscular specimens grouped together, indicating that all isolates were T. multiceps. There was no significant variation in the cox2 gene sequences within Coenurus although interspecies differences were obvious in Taenia. Therefore, cox2 gene was suitable as a molecular marker to study the intraspecific variation of T.multiceps. The results of the present study paid a solid foundation for molecular diagnosis of coenurosis.关键词
多头蚴/山羊/cox2基因/种系发育关系Key words
Coenurus/goats/cox2 gene/phylogenetic relationship分类
农业科技引用本文复制引用
郝桂英,杨应东,杨光友..四川省山羊多头蚴线粒体cox2基因序列测定及种系发育分析[J].中国动物传染病学报,2015,(1):54-59,6.基金项目
教育部长江学者和创新团队发展计划项目 （）
