中国动物传染病学报2015，Vol.23Issue(3)：7-11,5.
猪伪狂犬病毒变异株单克隆抗体的制备与鉴定
PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST PORCINE PSEUDORABIES VIRUS VARIANTS
摘要
Abstract
In order to generate the monoclonal antibodies (MAbs) against a variant of Porcine pseudorabies virus (PRV), BALB/c mice were immunized with purifi ed and inactivated PRV JS-2012 strain. Two hybridomas secreting PRV specifi c MAbs were obtained and designated as 4D8 and 4F10, respectively. Both MAbs belonged to IgG1 subgroup and hadκ light chains. The asceticfl uids containing either 4D8 or 4F10 showed indirect immunofl uorescence assay (IFA) titers at 1:51200. Both MAbs reacted specifi cally with PRV in IFA and immunohistochemical (IHC). In Western blot, MAb 4D8 reacted with 60 kDa protein of PRV while MAb 4F10 showed no obvious reactions with PRV protein, suggesting that MAb 4F10 might react with a conformational epitope. The availability of PRV MAbs was important for further research of antigenic variation and diagnosis.关键词
伪狂犬病毒/单克隆抗体/间接免疫荧光检测/免疫组化鉴定Key words
Pseudorabies virus/monoclonal antibody/indirect immunofl uorescence assay (IFA)/immunohistochemical (IHC)分类
农业科技引用本文复制引用
刘飞,童武,杨莘,郑浩,李国新,梁超,田青,童光志..猪伪狂犬病毒变异株单克隆抗体的制备与鉴定[J].中国动物传染病学报,2015,23(3):7-11,5.基金项目
上海市自然科学基金项目(14ZR1448900) （14ZR1448900）
中央级公益性科研院所基本科研业务费专项资金项目(2014JB02) （2014JB02）
