中国动物传染病学报2015，Vol.23Issue(3)：50-54,5.
鸭甲肝病毒与新型呼肠孤病毒复合RT-PCR检测方法的建立
MULTIPLEX RT-PCR FOR DETECTION OF DUCK HEPATITIS A VIRUS AND NOVEL DUCK REOVIRUS
摘要
Abstract
A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay for detecting Duck hepatitis A virus (DHAV) and Novel duck reovirus (NDRV) was developed using two primers based on 3D gene of DHAV and S3 gene of NDRV. The detection limit of the RT-PCR assay was 100 copies for both viruses. Mean while, the method had no reaction with Muscovy duck reovirus (MDRV), Duck plague virus (DPV), Duck Tembusu virus (DTMUV) and Duck paramyxo virus (NDV). The results of testing clinical samples showed the assay was a useful technique for differential detection of DHAV and NDRV.关键词
鸭甲肝病毒/新型鸭呼肠孤病毒/复合RT-PCR/鉴别诊断Key words
Duck hepatitis A virus/Novel duck reovirus/multiplex reverse transcription polymerase chain reaction/differential diagnosis分类
农业科技引用本文复制引用
孙晓军,王晓艺,韩宏宇,迟海华,杨宏禹,于可响..鸭甲肝病毒与新型呼肠孤病毒复合RT-PCR检测方法的建立[J].中国动物传染病学报,2015,23(3):50-54,5.基金项目
山东省海外高层次人才(泰山学者)引进计划 （泰山学者）
山东省现代农业产业技术体系家禽创新团队计划(SDAIT-13-011-01) （SDAIT-13-011-01）
公益性行业(农业)科研专项(201003012) （农业）
