中国体外循环杂志Issue(2)：113-118,122,7.DOI:10.13498/j.cnki.chin.j.ecc.2015.02.12
褪黑素受体对大鼠心肌缺血再灌注损伤的调控及其机制研究
The role of melatonin receptor in the protective effects of melatonin againstmyo-cardial ischemia reperfusion injury in rats
摘要
Abstract
[ Abstract]:Objective To investigate the protective effect of melatonin ( Mel) on myocardial ischemia/reperfusion ( MI/R) in⁃jury and the influence of melatonin membrane receptor ( MR) on MI/R injury. Methods Eighty male Sprague-Dawley rats were subjected to myocardial ischemia/reperfusion ( MI/R, I 30 min, R 6 h) operation and randomly divided into 4 groups: Sham, MI/R+V, MI/R+Mel and MI/R+Mel+Luz ( Luzindole, the specific antagonist of MR) . The cardiac function 72 h after reperfu⁃sion, oxidative stress damage related indicators, apoptotic index, infarct size, MR expression, SIRT1 expression, Ac-Foxo1 expres⁃sion were detected. Results Compared with the MI/R+V group, melatonin treatment group showed improved left ventricular ejection fraction (LVEF) (65.18±5.863) vs (47.37±4.201), ( P<0.01) and left ventricular fractional shortening (LVFS) (36.19±3.299) vs (22.80±0.8881), ( P<0.01), decreased myocardium superoxide generation (2.881±0.1908) RLU/(mg·s) vs (3.955±0.3022) RLU/(mg·s), ( P<0.01), malondialdehyde(MDA) level (269.1±11.24) pmol/mg vs (412.7±24.39) pmol/mg, ( P<0.01) and gp91phox expression (3.404±0.2440) vs (4.388±0.1463), ( P <0.01), up-regulated SIRT1 expression(0.8033±0.05357) vs(0.3313±0.04337), ( P<0.01), down-regulated Ac-Foxol expression(0.2393±0.01440) vs (0.3536±0.01384), ( P<0.01). More⁃over, Luz exposure significantly blocked melatonin's cardioprotective effect (all P<0.01). Conclusion The experiment showed that Mel pro⁃tected the heart against MI/R injury by reducing oxidative stress damage via activation of SIRT1 signaling in a MR dependent manner.关键词
褪黑素/褪黑素受体/心肌缺血再灌注损伤/沉默信息转录调控因子1/心肌保护Key words
Melatonin/Melatonin receptor/Myocardial ischemia/reperfusion injury/SIRT1/Cardioprotection引用本文复制引用
赵国龙,张秋芳,翟蒙恩,于立明,高文丽,金振晓,段维勋,俞世强,王云..褪黑素受体对大鼠心肌缺血再灌注损伤的调控及其机制研究[J].中国体外循环杂志,2015,(2):113-118,122,7.基金项目
国家自然科学基金（81470415）；国家自然科学基金（81470411）；陕西省科技统筹创新工程计划 （）
