中国斜视与小儿眼科杂志Issue(4)：12-14,3.
Ang-2在高氧诱导新生鼠ROP动物模型中的表达及意义
The expression of Ang-2 in the retina of hyperoxia-induced model of retinopathy
赵淼焱 1郭惠平 1闫丽1
作者信息
- 1. 100073首都医科大学电力教学医院眼科
- 折叠
摘要
Abstract
Objective To investigate the expression of Ang-2 in the retina of hyperoxia-induced model of retinopathy and to evaluate the effect of Ang-2 in retinal neovascularization. Methods Sixty newborn SD rats were randomly divided into hyperoxia and normoxia control groups.30 rats in hyperoxia group were exposed to hyperoxia for 5 days,and subsequently were moved into room air.Another 30 rats were raised in room air.Rats of postnatal day 17 were sacrificed. Then eyes were embedded in paraffin and frozen sections were stained with immunohisto-chemistry to investigate the expression of ang-2. Also, the number of new vascular cell nuclei extending into the internal limiting membrane was counted to assess retinal neovascularization. Results Retinal neovascularization reached maximum on the 17th day.The highest number of neovascular nuclei extending into the internal limiting membrane was 34.78±4.38 compared with that of the same age of control group,1.56±1.64,( <0.001).The expres-sion of ang-2 in hyperoxia group was the strongest in the retina on the 17th day,and the optical density value(OD value)was 117.25±10.67,significantly higher than that of the age-matched control group,83.29±9.15,( < 0.001). Conclusions The retinal neovascularization was in accordance with the change of ang-2 expression, suggesting that there is a close relationship between the expression of ang-2 and retinal neovascularization.关键词
视网膜病早产儿/血管生成素-2新生血管/疾病模型/动物Key words
retinopathy/permaturity/angiopoietin-2/neovascularization/model animal引用本文复制引用
赵淼焱,郭惠平,闫丽..Ang-2在高氧诱导新生鼠ROP动物模型中的表达及意义[J].中国斜视与小儿眼科杂志,2014,(4):12-14,3.
