中国循证儿科杂志Issue(3)：211-215,5.DOI:10.3969/j.issn.1673-5501.2014.03.011
高压氧对缺氧缺血1周内新生大鼠脑皮质细胞线粒体膜电势的影响
Effect of hyperbaric oxygenation on mitochondrial membrane potential of cortex neuronal cells of neonatal rats in the first week after hypoxic ischemic brain damage
摘要
Abstract
Objective The initial insult of hypoxic-ischemic( HI)brain damage( HIBD)is the deprivation of oxygen( O2 ) to the brain cells,followed by a cascade of brain cell damage including mitochondrial dysfunction. Theoretically,hyperbaric oxygenation( HBO)could affect the recovery of mitochondrial function in HIBD by greatly increasing the O2 delivery diffusion gradient. The objective of this study was to prove the hypothesis that HBO may reduce HI-induced brain injury via affecting brain cell mitochondrial function,and to understand the changing patterns of mitochondrial function following HBO treatment in the first week after HI. Methods In the present study,HIBD rat model and flow cytometer were used to explore the change of ΔΨm,the indicator of mitochondrial function of cortex neuronal cells of neonatal rats after HIBD. Neonatal Sprague Dawley( SD)rat pups were randomly divided into normal control,HIBD,and HIBD+HBO groups. The end of HI was considered to be 0 h time point. The HBO treatment was given at 0h time point,and then once a day for consecutive 7 days( in 24 h intervals). Animals were euthanized at 0,2,4,6,12 h time points(in order to study theΔΨm changes at the very early stage after a single dose of HBO treatment),and at 2,3,4,5,6,and 7 d time points( in order to study the ΔΨm changes after a series of HBO treatment). Results The change of ΔΨm of the ipsilateral cortex in both HIBD and HIBD+HBO groups showed fluctuating change pattern. Within 2 h to 12 h after HI insult,ΔΨm of HIBD group recovered to some extent,but ΔΨm of HIBD+HBO group recovered to almost normal level. A secondary drop of ΔΨm was observed in both groups at 1 -4 d after HI insult. The secondary drop of HIBD+HBO group was more severe than that of HIBD group. There was a secondary recovery of ΔΨm observed in HIBD+HBO group in 5-7 d after HI insult,but not in HIBD group. TheΔΨm of HIBD+HBO group recovered again to almost normal level at 6 d time point. TheΔΨm of HIBD group in 2-7 d after HI stayed at low level,showing slowly decreasing tendency. Conclusion HBO in the early stage after HI might not be a good therapy to improve the mitochondrial function in the cerebral cortex. The secondary recovery observed in HIBD + HBO group indicated that HBO treatment may protect HI-induced brain damage by improving neural cell mitochondrial function in the cerebral cortex during sub-acute stage after HI.关键词
高压氧/缺氧缺血/一周内/脑/线粒体功能/新生大鼠Key words
Hyperbaric oxygenation/Hypoxic-ischemic/The first week/Brain/Mitochondrial function/Neonatal rat引用本文复制引用
张小春,黑明燕,罗娅丽,李媛媛,戴津津..高压氧对缺氧缺血1周内新生大鼠脑皮质细胞线粒体膜电势的影响[J].中国循证儿科杂志,2014,(3):211-215,5.基金项目
湖南省自然科学基金课题11JJ6067 （）
