中国循证儿科杂志Issue(5)：359-364,6.DOI:10.3969/j.issn.1673-5501.2014.05.007
Illumina高通量测序技术分析早产儿出生后肠道菌群变化的初步研究
Changes of postnatal gut microbiota in preterm infants using Illumina high-throughput sequencing technology
陈娜 1杨毅 1张澜 1方剑火 2郎继东 2曹云 1田埂2
作者信息
- 1. 复旦大学附属儿科医院 上海，201102
- 2. 清华大学医学院 北京，100084
- 折叠
摘要
Abstract
Objective To explore changes of postnatal gut microbiota in preterm infants in NICU and its association with neonatal sepsis. Methods preterm infants hospitalized in NICU of Childrenˊs Hospital of Fudan University were enrolled in the study. Fecal samples were collected at day 1,7,14 and 21 after birth,respectively. Illumina high-throughput sequencing techno-logy was used to sequence 16S rRNA-V3 hypervariable region of all microbes in 7 fecal samples of 3 recruited subjects. MG-RAST V3. 3. 6 was used to analyze and calculate the numbers of sequences and operational taxonomic units(OTUs)for each sample,then the species abundance and distribution were analyzed and followed by cluster analysis. Results Seven samples had been analyzed. Three subjects had an average gestational age of(31. 3 ± 0. 8)weeks and an average birth weight of(1 540 ± 144)g. They all received antibiotic administration after birth. Maternal antibiotic exposure occured in 1 case. Another one case developed SIRS during hospitalization. ①The rarefaction curves showed that adequate sequencing depth was achieved. Analysis of species abundance showed that the OTUs number ranged from 381 to 608 indicating high microbial diversity. Meanwhile,the microbial diversity had a positive correlation with the postnatal age. ②Eighteen phyla were detected from all samples. Actinobacteria, Bacteriodetes,Firmicutes and Proteobacteria were predominant in all samples. However,the dominant phylum of case 1 belonged to Proteobacteria,with a percentage of 97. 5%(day 14)and 49. 1%(day 21),while Actinobacteria predominated in day-7 sample of case 2,with a percentage of 99. 5%. ③172 families were detected altogether,63 of them were detected in all samples. Ten families were in higher relative abundance. In case 2,Corynebacteria occupied 97. 9% in day-7 sample,and Staphylococcaceae occupied a higher proportion in day-21 sample(27. 2%)than the other two samples. ④The cluster analysis showed high similarity of intestinal microflora in different time points of one subject. Conclusion Gut microbial colonization and development in preterm infants in NICU were altered by various factors. prolonged broad-spectrum antibiotics at prenatal and early postnatal age might profoundly decrease microbial diversity and affect microbial colonisation. Microbiota was less diverse from birth in infants who developed sepsis. There may be a microbiome predominanted of pathogens that may be associated with sepsis in preterm infants.关键词
早产儿/肠道菌群/败血症/高通量测序Key words
preterm infants/Gut microbiota/Sepsis/High-throughput sequence引用本文复制引用
陈娜,杨毅,张澜,方剑火,郎继东,曹云,田埂..Illumina高通量测序技术分析早产儿出生后肠道菌群变化的初步研究[J].中国循证儿科杂志,2014,(5):359-364,6.
