中国烟草学报Issue(3)：96-101,6.DOI:10.3969/j.issn.1004-5708.2014.03.016
烟草靶斑病菌（Rhizoctonia solani）SRAP-PCR 体系建立及优化
Establishment and optimization of SRAP-PCR reaction system for Rhizoctonia solanifrom tobacco target spot
摘要
Abstract
SRAP primer pairs were screened for polymorphism using DNA of Rhizoctonia solaniisolates YC-9, LJT-8 and QYS-7 as templates. An orthogonal design of L16(45) was used to optimize SRAP-PCR reaction system forR. solaniof tobacco with 5 factors, namely Mg2+, dNTPs, primers, Taq DNA polymerase and template DNA. Results showed that a total of 13 polymorphic SRAP primer pairs were screened out of 100 SRAP primer pairs, and a suitable SRAP-PCR reaction system for Rhizoctonia solanifrom tobacco target spot was 2.0 mmol.L-1 Mg2+, 200 mmol.L-1 dNTPs, 0.8U Taq DNA polymerase, 140 mmol.L-1 primer pairs, 20 ng template DNA and 1×PCR buffer. In addition, each factor in SRAP-PCR reaction system had different effects on amplified patterns in descending order of Taq DNA polymerase> primer>Mg2+> dNTPs=DNA.关键词
烟草靶斑病菌/正交试验设计/反应体系优化/引物筛选Key words
Rhizoctonia solani from tobacco target spot/orthogonal experiment design/optimization of reaction system/primer screening分类
农业科技引用本文复制引用
赵艳琴,吴元华,赵秀香,安梦楠,陈建光..烟草靶斑病菌（Rhizoctonia solani）SRAP-PCR 体系建立及优化[J].中国烟草学报,2014,(3):96-101,6.基金项目
国家烟草专卖局科技攻关项目[国烟办综(2010)182号]；辽宁省烟草专卖局科技攻关项目[辽烟计（2010）86号] （2010）
