中国烟草学报Issue(4)：94-100,7.DOI:10.3969/j.issn.1004-5708.2014.04.018
烟草NtSnRK2.1基因的克隆及其在非生物胁迫条件下的表达
Cloning and expression ofSnRK2.1 gene under abiotic stress
摘要
Abstract
One EST ofSnRK2was screened from glandular trichome cDNA library of tobacco. Based on the EST sequence,NtSnRK2.1 was isolated from tobacco (Nicotiana tabacum L.) byin silicocloning and RT-PCR.NtSnRK2.1 includes an open reading frame (ORF) of 1017 bp and encodes 338 deduced amino acid residues (AAR) with a calculated molecular mass of 43 kDa and a predicted pI of 5.78. Scansite analysis indicated that NtSnRK2.1 contained potential serine/threonine protein kinase activities like other SnRK2 family members. Phylogenetic analysis suggestedNtSnRK2.1 was high homologous to its orthologous genes from Arabidopsis, rice and wheat, which were also induced by abiotic stresses. Reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qRT-PCR) was used to determine expression patterns ofNtSnRK2.1 in tobacco. Results revealed thatNtSnRK2.1 expressed most strongly in tobacco roots, more in leaves, and marginally in stems. Expression patterns under abiotic stress responses suggested thatNtSnRK2.1 was involved in response to NaCl, PEG, cold stresses and ABA treatment, with significant different responsive profiles. The sensitivity degrees of NtSnRK2.1 responding to four treatments was in the order of hyperosmolality> high salinity> low temperature (4℃)> abscisic acid.关键词
普通烟草/NtSnRK2.1/非生物胁迫/克隆/表达分析Key words
Nicotiana tabacum/NtSnRK2.1/abiotic stress/cloning/expression analysis分类
生物科学引用本文复制引用
张洪映,贾宏昉,张松涛,杨永霞,崔红..烟草NtSnRK2.1基因的克隆及其在非生物胁迫条件下的表达[J].中国烟草学报,2014,(4):94-100,7.基金项目
中国烟草总公司特色优质烟叶开发重大专项浓香型项目（110201101001 TS-01），国家烟草局资助 （）
