中国烟草学报Issue(5)：103-109,7.DOI:10.3969/j.issn.1004-5708.2014.05.017
烟草过氧化氢酶基因CAT1的克隆及表达特征分析
Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L
摘要
Abstract
The full-length cDNA of Catalase 1 (CAT1) from Nicotiana tabacum var. NC89 was cloned based on specific primers designed according to CAT1 mRNA sequences in other plants. An amino acid sequence alignment indicated that CAT1 contains 492 amino acid residues. Phylogenetic analysis showed that NC89 CAT1 shares high similarity with gene from N. benthamiana. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed that CAT1 is highly expressed in leaf and calyx, while less expressed in roots and seeds. The expression of NC89 CAT1 was enhanced by abiotic and biotic stressors, such as mechanical damage, osmotic pressure, low temperature, high temperature, drought, and TMV infection, whereas it was inhibited by ultraviolet irradiation. It was suggested that CAT1 plays an important role in growth, development, and interplay of abiotic and biotic stresses in plant.关键词
过氧化氢酶/烟草/表达分析/生物胁迫/非生物胁迫Key words
catalase/nicotiana tabacum/expression analysis/biotic stress/abiotic stress分类
生物科学引用本文复制引用
王升平,宋玉川,杨金广,战徊旭,申莉莉,钱玉梅,武侠,王凤龙,李锡宏,陈晓明..烟草过氧化氢酶基因CAT1的克隆及表达特征分析[J].中国烟草学报,2014,(5):103-109,7.基金项目
国家烟草专卖局全国烟草有害生物调查研究(110200902065)；湖北省烟草公司科技项目（027Y2013-006）；云南省烟草公司科技项目（2013YN37）；遵义市烟草公司科技项目（2013-11）；山东省“泰山学者”建设工程专项经费；中国农业科学院基本科研业务费预算增量项目 （110200902065）
