中国烟草学报Issue(3)：100-106,7.
烟草NCED3基因的克隆及其干旱胁迫表达分析
Cloning ofNCED3 gene in Nicotiana tabacum and analysis of its drought stress-induced expression
摘要
Abstract
In order to reveal the function ofNCED3 gene in tobacco plant growth and development, two copies (NCED3-1andNCED3-2) of full-length cDNA sequence of tobacco nine-cis-epoxycarotenoid dioxygenase3 were cloned from K326 by homology cloning strategy. Sequences analysis showed thatNCED3-1and NCED3-2 contained 1842-bp and 1830-bp open reading frame (ORF ) and coded 613 and 609 amino acid residues, and the calculated molecular mass was 68177.8Da and 67754.2Da with the theoretical isoelectric point (pI) of 7.22 and 7.28, respectively. Protein hydrophobicity, transmembrane domains and signal peptide analysis indicated that the protein might be transmembrne and hydrophilic, and located on mitochondrial membrane. Secondary structure prediction results showed that they were mainly made up by beta turns and random coil. Tertiary structure prediction results displayed that NCED3-1 showed high similarity with NCED3-2. Expression patterns under abiotic stress responses suggested that PEG6000 could induce expression ofNCED3 and accumulation of ABA. Expression of NCED3 and ABA accumulation rate both increased before 12h treatment, and then decreased gradually. These results provided some basis for drought function analysis ofNCED3.关键词
烟草/NCED3基因/克隆/序列分析/表达/ABAKey words
tobacco/NCED3/cloning/sequence analysis/expression/ABA引用本文复制引用
牛志强,刘国顺,师婷婷,张松涛,贾宏昉,张洪映,崔红,杨永霞..烟草NCED3基因的克隆及其干旱胁迫表达分析[J].中国烟草学报,2015,(3):100-106,7.基金项目
河南省教育厅自然科学研究项目 （）
中国烟草总公司特色优质烟叶开发重大专项浓香型项目（No.110201101001 TS-01) （）
