中国药房Issue(18)：2569-2570,2.DOI:10.6039/j.issn.1001-0408.2015.18.43
HPLC法测定消核糖浆中芍药苷的含量
Content Determination of Paeoniflorin in Xiaohe Syrup by HPLC
周儒兵 1杨帆 2董晓蓉3
作者信息
- 1. 重庆市肿瘤研究所，重庆 400030
- 2. 重庆市食品药品检验所，重庆 401121
- 3. 重庆市药物过程与质量控制工程技术研究中心，重庆 401121
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for determining the content of paeoniflorin in Xiaohe syrup. METHODS:HPLC method was adopted. The Waters SunFire C18 column was used with the mobile phase of acetonitrile-0.1% phosphoric acid(12∶88, V/V)at the flow rate of 1.2 ml/min;the detection wavelength was 230 nm with column temperature at 30℃. Sample size was 10μl . R E-SULTS:There was a good linear relationship between the amount of paeoniflorin and peak area in the range of 0.061 0-1 . 2 2 0 8 μg (r=0.999 9). The RSDs of precision,stability and reproducibility tests were all less than 2% and the average recovery was 98.77%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,rapid and accurate and can be used for the content deter-mination of paeoniflorin in Xiaohe Syrup.关键词
消核糖浆/芍药苷/高效液相色谱法/含量测定Key words
Xiaohe syrup/Paeoniflorin/HPLC/Content determination分类
医药卫生引用本文复制引用
周儒兵,杨帆,董晓蓉..HPLC法测定消核糖浆中芍药苷的含量[J].中国药房,2015,(18):2569-2570,2.
