中国药房Issue(19)：2614-2616,2617,4.DOI:10.6039/j.issn.1001-0408.2015.19.07
肠激安胶囊对腹泻性肠易激综合征模型大鼠腹痛的影响及分子机制研究Δ
Study on the Effect of Changji’an Capsules on Abdominal Pain in Diarrhea-predominant Irritable Bowel Syndrome Model Rats and Its Molecular Mechanism
摘要
Abstract
OBJECTIVE:To investigate the effect of Changji’an capsules on abdominal pain and the molecular mechanism re-lated to calcitoningene-related peptide(CGRP)and corticosterone(CORT)in diarrhea-predominant irratable bowel syndrome(IBS-D)model rats. METHODS:After the rat models of IBS-D were established by the method of separation of breast mick combined with stimulation with acetic acid,the rats were randomly divided into model group(isometric normal saline),pinaverium bromide group(0.018 g/kg),and Changji’an capsules high,medium and low dose groups(2.812 g/kg,1.406 g/kg and 0.703 g/kg),and another SD rats were included in the normal control group(isometric normal saline). The drugs were given to the rats once a day for consecutive 14 d,ig. Injection of normal saline method was adopted to determine the rat’s sensibility to abdominal pain. The en-zyme-linked immunosorbent assay(ELISA)was adopted to determine the content of CORT in the serum of the rats. Subjected to re-verse transcription polymerse chain reaction(RT-PCR)was adopted to determine the expression of CGRP mRNA in the hypothala-mus and colon tissues of the rats. RESULTS:Compared with normal control group,threshold values of arching the back and stick-ing out the abdomen were decreased,the content of CORT in serum and expression of CGRP mRNA in the hypothalamus and co-lon tissues in model group were increased,with significant difference(P<0.01). Compared with model group,threshold values of arching the back and sticking out the abdomen were increased,the content of CORT in serum and expression of CGRP mRNA in the hypothalamus and colon tissues in pinaverium bromide group and Changji’an capsules high and medium dose groups were de-creased;the threshold value of arching the back in Changji’an capsules low dose group were increased,with significant difference (P<0.01 or P<0.05). CONCLUSIONS:Changji’an capsules can improve the abdominal pain in rats with IBS-D by a mechanism that may be related to the decrease in the expression of CGRP mRNA in the hypothalamus and colon tissues and the reduc-tion of the content of CORT in serum.关键词
肠激安胶囊/腹泻性肠易激综合征/降钙素基因相关肽/皮质酮/下丘脑/结肠/大鼠Key words
Changji’an capsules/Diarrhea-predominant ir-ritable bowel syndrome/Calcitonin gene-related peptide/Corti-costerone/Hypothalamus/Colon/Rats分类
医药卫生引用本文复制引用
房财富,唐洪梅,廖小红,黄红兵,刘韬..肠激安胶囊对腹泻性肠易激综合征模型大鼠腹痛的影响及分子机制研究Δ[J].中国药房,2015,(19):2614-2616,2617,4.基金项目
国家自然科学基金资助项目（No.81073160）；广东省自然科学基金资助项目 （）
