摘要
Abstract
OBJECTIVE:To study the mechanism of apoptosis of human liver cancer SMMC7721 cells induced by tetrandrine (Tet). METHODS:MTT assay was used to test the cells activity and calculate the inhibition rate after the cells were cultured by 4, 6,8 and 10 mg/L Tet for 24,48 and 72 h. Flow cytometry was used to test the cells apoptotic ratio after the cells were cultured by 6 mg/L Tet for 24,48 and 72 h;Mitochondrial membrane potential (JC-1) was examined under an inverted fluorescence micro-scope;colorimetric method was used to detect the Caspase-3 activity;the protein expressions of Cyto-C and Pro-caspase-3 were ex-amined by Western blot. All of the tests were operated with blank control (normal culture medium or cultivated for 0 h). RE-SULTS:There was an obvious inhibition on the proliferation of SMMC7721 cells in a time- and dose-dependent manner after cells were cultured by 4,6,8 and 10 mg/L Tet for 24,48 and 72 h. Compared with the blank control,after cells were cultured by 6 mg/L Tet for 24,48 and 72 h,the cells apoptotic ratio was increased,JC-1 was decreased in a dose-dependent manner. There was an in-crease in the Caspase-3 activity after cells were cultured by 6 mg/L Tet for 24 and 48 h. After cells were cultured by 6 mg/L Tet for 24 h,the Cyto-C protein expression was strengthened,and Pro-caspase-3 protein expression was decreased,with statistical differ-ences (P<0.05). CONCLUSIONS:Tet treatment can induce the apoptosis of SMMC7721 cells by a mechanism that is related to the activation of mitochondrial apoptotic pathway.关键词
汉防己甲素/人肝癌SMMC7721细胞/凋亡/线粒体膜电位Key words
Tetrandrine/Human liver cancer SMMC7721 cells/Apoptosis/Mitochondrial transmembrane potential分类
医药卫生
