中国药房Issue(21)：2971-2974,4.DOI:10.6039/j.issn.1001-0408.2015.21.33
地龙的HPLC特征图谱研究
Study on the HPLC Characteristic Chromatogram of Pheretima
摘要
Abstract
OBJECTIVE:To establish the HPLC characteristic chromatogram of pheretima,and compare the differences of the main ingredient contents of Guangdong pheretima and Shanghai pheretima and the chromatogram differences among pheretima and 3 other animal drugs. METHODS:Pheretima HPLC characteristic chromatogram method was adopted to determine the characteris-tic chromatograms of 16 Guangdong pheretima,8 Shanghai pheretima,3 eupolyphaga,3 hirudo and 3 catharsius. Similarity evalua-tion and t test were used to analyze the differences of chromatogram data of 5 animal drugs. RESULTS:The established HPLC char-acteristic chromatogram method firstly identified 11 common characteristic peaks,including 6 nucleosides,4 nucleobase and 1 ami-no acid;and it could be used for the identification of pheretima from eupolyphaga,hirudo and catharsius;the differences of main ingredient contents in the characteristic chromatogram of Guangdong pheretima and Shanghai pheretima were firstly studied. The contents of xanthine and adenosine in Guangdong pheretima were higher than Shanghai pheretima,while the contents of uridine, guanosine and 2′-deoxy guanosine in Shanghai pheretima were higher than Guangdong pheretima. A new index S,calculated by these 5 constituents,was successfully applied to distinguish the 2 kinds of pheretima. CONCLUSIONS:The characteristic chro-matogram can be used for the identification of pheretima,and can provide reference for the pharmacodynamic differences study of Guangdong pheretima and Shanghai pheretima.关键词
地龙/广地龙/沪地龙/高效液相色谱法/特征图谱Key words
Pheretima/Guangdong pheretima/Shanghai pheretima/HPLC/Characteristic chromatogram分类
医药卫生引用本文复制引用
黄帅,徐风,杨平,杨雪雁,刘广学,尚明英,王璇,蔡少青..地龙的HPLC特征图谱研究[J].中国药房,2015,(21):2971-2974,4.基金项目
国家科技重大专项课题--重大新药创制 （）
