中国药房Issue(21)：2983-2985,2986,4.DOI:10.6039/j.issn.1001-0408.2015.21.37
HPLC法测定马钱子碱纳米结构脂质载体的主药含量及包封率Δ
Determination of the Main Content and Entrapment Efficiency in the Brucine Nanostructured Lipid Carrier by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for determining the main contents and entrapment efficiency in the bruc-ine nanostructured lipid carrier(NLC). METHODS:HPLC was adopted to determine the main content,sephadex gel filtration meth-od was employed to separate free drug in brucine NLC to determine the entrapment efficiency. The column was Dikma C18 with the mobile phase of mobile phase A(methanol)-mobile phase B [water-acetic acid-triethylamine(230∶2.4∶0.3,V/V/V)](30∶70,V/V)at the flow rate of 1 ml/min,the detection wavelength was 265 nm,volume was 10 μl and temperature was 30 ℃. RESULTS:The linear range of brucine was 4.00-80.00μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were≤1.67%;av-erage recoveries of content determination and sephadex gel filtration method were respectively 99.66%(RSD=0.45%,n=9) and 99.75%(RSD=1.74%,n=9);and the average entrapment efficiency was 69.92%. CONCLUSIONS:The method is simple,re-producible and efficient,and can be used for the determination of main contents and entrapment efficiency in brucine NLC.关键词
马钱子碱/纳米结构脂质载体/高效液相色谱法/葡聚糖凝胶柱过滤法/含量/包封率Key words
Brucine/Nanostructured lipid carrier/HPLC/Sephadex gel filtration method/Content/Entrapment efficiency分类
医药卫生引用本文复制引用
管庆霞,华晓丹,张喜武,李秋红,李伟男,赵宇巍..HPLC法测定马钱子碱纳米结构脂质载体的主药含量及包封率Δ[J].中国药房,2015,(21):2983-2985,2986,4.基金项目
黑龙江省教育厅科学技术研究项目 （）
