中国医科大学学报Issue(5)：441-445,5.
降脂抗凝联合作用对兔激素性股骨头坏死骨细胞凋亡的影响
Impact of Lipid Lowering and Anti-coagulant Combined Application on the Femoral Bone Cell Apoptosis in Rabbit with Steroid-induced Osteonecrosis
摘要
Abstract
Objective To investigate the combined effects of lipid lowering and anti-coagulant on the femoral bone cell apoptosis in rabbit with ste-roid-induced osteonecrosis. Methods A total of 44 female Japanese white rabbits were randomly divided into three groups:normal saline group (CTR group,12 rabbits),steroid-induced osteonecrois of femoral group(SIONF group,16 rabbits),and atorvastatin warfarin treated group(AW group,16 rabbits). Intravenous injection of endotoxin of E.coli(10μg·kg-1)and intramuscular injection of methylprednisolone(20 mg·kg-1)were used to establish the SIONF rabbit model in SIONF group and AW group. CTR group was only administrated with saline. AW group was given atorv-astatin 2.5 mg·kg-1·d-1 and warfarin 1.0 mg·kg-1·d-1 by oral. Random sampling of two animals before the experiment two,four,six weeks after the experiment,and at the end of experiment(the 8th week),the venous blood was collected for testing serum total cholesterol(Tch),low density lipo-protein(LDL),prothrombin time(PT),tissue-type plasminogen activation time(t-PA),and taken bilateral hip X-rays. Then,all animals were scarified,and the femoral head bone cell apoptosis was determined. Results From the sixth week to the end of the experiment,Tch and LDL were slightly increased in CTR group and AW group,but no significant difference was found between the two groups. Tch and LDL were significantly in-creased in SIONF group than CTR group and AW group(P<0.05). From the beginning of fourth week,the PT and t-PA was significantly prolonged in AW group,compared to CTR group and SIONF group(P<0.05),and conversely the PT and t-PA was shortened in SIONF group compared to CTR group(P<0.05).At the end of experiment,the X-rays showed that the structure of femoral head in CTR group was normal,but the femoral head surface in SIONF group was rough and cartilage collapse with an incidence of 44%.In AW group,the femoral head surface was slightly rough and trabecular fuzzy but significantly reduced as compared with SIONF group,and the incidence was 25%. From the beginning of fourth week,the bone cell AI in AW group began to increase till end of the experiment(peaked at 39),which was significantly higher than CTR group(23)(P<0.05),but significantly lower than SIONF group(66)(P<0.05). Conclusion The combined use of lipid-lowering and anti-coagulant can signifi-cantly reduce the occurrence of SIONF bone cell apoptosis in rabbit,and the mechanism may be concerned with the improved high fat and high coag-ulation status.关键词
骨细胞/凋亡/糖皮质激素/兔/阿托伐他汀/华法林Key words
bone cell/apoptosis/glucocorticoids/rabbit/atorvastatin/warfarin分类
医药卫生引用本文复制引用
李挺松,肖增明,黄秋嫔,劳山,杨劲松,罗高斌..降脂抗凝联合作用对兔激素性股骨头坏死骨细胞凋亡的影响[J].中国医科大学学报,2014,(5):441-445,5.基金项目
广西自然科学基金（2013GXNSFAA019164）；广西高校资助科研项目 （）
