中国医科大学学报Issue(2)：105-108,4.
胰岛素抵抗肥胖大鼠体内增食欲素A与丝氨酸蛋白酶抑制剂及脂代谢的相关性研究
Correlation between Orexin A and Vaspin in Lipid Metabolism in Obese Rats with Insulin Resistance
摘要
Abstract
Objective To investigate the correlation between orexin A and the serine proteinase inhibitor vaspin in obese rats with insulin resis⁃tance(IR)induced by high⁃fat diet and elaborate the possible action mechanism of orexin involvement in fat metabolism in IR pathological process. Methods A total of 75 4⁃week⁃old male Sprague⁃Dawley rats were randomly divided into the normal dietary group(NC group,n=20)and the high⁃fat dietary group(HF group,n=55)to establish the model of obese rats with IR,the euglycemic insulin clamp technique was used to determine re⁃lated indicators of insulin resistance and lipid metabolism. The rats were treated with orexin A(1×10-8⁃1×10-6 mol/L)by hypodermic injection. The serum levels of orexin A and vaspin in rats were detected with enzyme linked immunosorbent assay. Results After 6 weeks of high⁃fat diet,the se⁃rum glucose,insulin,TC,TG and LDL⁃C were increased significantly in HF group than in NC group,while GIR60~120 in obese rats was decreased significantly[(16.31 ± 1.54)vs(30.22 ± 2.76)mg/(kg · min),P<0.05]. The serum vaspin level was increased 177.08%in HF group compared with NC group(P<0.05). With hypodermic injection of orexin A(1×10-8 mol/L,1×10-7 mol/L and 1×10-6 mol/L),the levels of serum vaspin in⁃creased 25.00%,68.75%,and 120.83%in NC group and increased 7.52%,24.06%,and 40.60%in HF group. There was a positive correlation be⁃tween vaspin and orexin A,glucose,insulin,TC,TG,and LDL⁃C(r1=0.482,P1=0.02,r2=0.515,P2=0.02,r3=0.303,P3=0.04,r4=0.388,P4=0.03,r5=0.255,P5=0.04,r6=0.253,P6=0.04)and a negative correlation between vaspin and HDL⁃C(r=-0.226,P=0.04)in obese rats with IR. Conclusion High⁃fat diet can induce insulin resistance and obesity in rats,and orexin A is closely correlated to vaspin in obese rats with insu⁃lin resistance. Orexin A increases serum vaspin expression and thus involves in onset of insulin resistance in obese rats.关键词
增食欲素A/vaspin/胰岛素抵抗/肥胖Key words
orexin A/vaspin/insulin resistance/obesity分类
医药卫生引用本文复制引用
马丽娜,赵玉岩,常晓岑..胰岛素抵抗肥胖大鼠体内增食欲素A与丝氨酸蛋白酶抑制剂及脂代谢的相关性研究[J].中国医科大学学报,2015,(2):105-108,4.基金项目
国家自然科学基金 （）
