中国医科大学学报Issue(2)：114-118,5.
凋亡抑制基因Livin对肺腺癌细胞A549增殖与耐药的作用
Effect of Livin on Proliferation and Multi-drug Resistance of Lung Cancer Cells A549
摘要
Abstract
Objective To study the effect of the inhibitor of apoptosis protein,Livin on proliferation and multi⁃drug resistance of lung adenocarcino⁃ma cells A549. Methods A549 cells were transfected with the eukaryotic expression vector pcDNA3.1⁃Livin. A549 cell clone with stable expres⁃sion of Livin was obtained through G418 screening. Expressions of Livin mRNA and protein in the transfected cells were respectively measured by re⁃verse transcription polymerase chain reaction(RT⁃PCR)and Western blot. The distribution of cell cycle phase was determined using flow cytometry. The level of P⁃gp mRNA and protein in A549 cells transfected with pcDNA3.1⁃Livin was detected by RT⁃PCR and Western blot. The analysis of multi⁃drug resistance of A549 treated with different chemotherapeutics was performed by MTT. Results The mRNA and protein expressions of Liv⁃in were both significantly increased in the transfected A549 cells. The flow cytometry analysis showed there was higher percentage of S phase and low⁃er percentage of G0/G1 phase in A549 cells transfected with pcDNA3.1⁃Livin. Compared with control groups,the expression of P⁃gp mRNA and pro⁃tein was increased in A549 cells transfected with pcDNA3.1⁃Livin,which showed a higher drug resistance and lower sensitivity to chemotherapic drugs such as ADM,MTX,CTX,and DDP(P<0.05). Conclusion Overexpression of Livin could enhance the proliferation of A549 cells,and high expression of P⁃gp caused by Livin could serve as one of the causes for multi⁃drug resistance in lung adenocarcinoma against chemotherapies.关键词
Livin/肺腺癌细胞/增殖/P-糖蛋白/多药耐药Key words
Livin/lung adenocarcinoma cells/proliferation/P-gp/multi-drug resistance分类
医药卫生引用本文复制引用
王金娜,王锦光,赵磊..凋亡抑制基因Livin对肺腺癌细胞A549增殖与耐药的作用[J].中国医科大学学报,2015,(2):114-118,5.基金项目
吴阶平医学基金会临床科研专项资助 （）
