中国医科大学学报Issue(2)：119-122,126,5.
食欲素A调控INS-1胰岛素瘤细胞的细胞增殖的分子机制
Molecular Mechanism for Regulation of INS-1 Rat Insulinoma Cell Proliferation by Orexin-A
摘要
Abstract
Objective To investigate the interference effects of orexin A on cell proliferation of the insulin⁃secreting beta⁃cell line(INS⁃1 cells) through the orexin receptor 1(OX1R)and the AKT/PKB signaling pathway. Methods INS⁃1 cells were exposed to different concentrations of orexin A in vitro,and treated with OX1R antagonist(SB334867),PI3K antagonist(wortmannin),or AKT antagonist(PF⁃04691502). The INS⁃1 cell proliferation and apoptosis,insulin secretion,OX1R protein activity and AKT phosphorylation level were determined. Results Orexin A(10-10 to 10-6 mol/L)stimulated the proliferation and activation of INS⁃1 cells,prevented apoptpsis,and increased insulin secretion. Additionally,AKT phosphorylation was stimulated by orexin A(10-10 to 10-6 mol/L). The OX1R antagonist SB334867(10-6 mol/L),the PI3K antagonist wortmannin (10-8 mol/L)and the AKT antagonist PF⁃04691502(10-6 mol/L)weakened the effects of orexin A. Conclusion Orexin A activated the AKT sig⁃naling pathway through the mediation of orexin A⁃OX1R,and promoted cell proliferation in INS⁃1 cells.关键词
增食欲素A/增食欲素受体1/细胞增殖/AKT信号通路/胰岛β细胞Key words
orexin A/orexin receptor 1/cell proliferation/AKT signaling pathway/pancreas isletβcells分类
医药卫生引用本文复制引用
孔媛,赵玉岩,温晶..食欲素A调控INS-1胰岛素瘤细胞的细胞增殖的分子机制[J].中国医科大学学报,2015,(2):119-122,126,5.基金项目
国家自然科学基金 （）
