中国医科大学学报Issue(2)：132-135,4.
DBC2在胃癌中的甲基化
Study on Methylation of DBC2 in Gastric Cancer
摘要
Abstract
Objective To detect the expression of DBC2 and the methylation status of its gene promoter region in gastric cancer,so as to analyze their correlation. Methods Gastric cancer tissues and adjacent normal tissues were collected from 92 cases as research subjects. The DBC2 mRNA expression and the methylation status of DBC2 gene promoter region in gastric cancer were detected by using RT⁃PCR and MS⁃PCR respectively. In addition,the correlation of DBC2 gene methylation status with clinicopathological parameters was analyzed. Results In comparison with adjacent normal tissues,gastric cancer tissues displayed a low expression level of DBC2mRNA and a high methylation level of gene promoter region. Besides, the methylation level was significantly correlated with tumor invasion depth,differentiation and TNM staging(P<0.05). With deep invasion,poor differentiation or higher TNM staging,methylation level of gene promoter region was more significant in gastric cancer tissues. Conclusion High methylation of DBC2 gene promoter region may be a significant contributing factor leading to low DBC2 expression.关键词
胃癌/甲基化/DBC2Key words
gastric cancer/methylation/DBC2分类
医药卫生引用本文复制引用
王红岩,刘峥嵘,赵晓丹,谢锋..DBC2在胃癌中的甲基化[J].中国医科大学学报,2015,(2):132-135,4.基金项目
辽宁省自然科学基金（201102108）；沈阳市科技计划项目 （）
