中国医科大学学报Issue(2)：160-163,188,5.
甲基化芯片检测APP/PS1双转基因小鼠基因组DNA甲基化分布
A Genome-wide Analysis of DNA Methylation in APP/PS1 Transgenic Mice by Methylation Microarrays
摘要
Abstract
Objective To investigate the distribution pattern of genome⁃wide CpG methylation in the animal model of APP/PS1 transgenic mice with Alzheimer′s disease(AD). Methods This study investigated the genome⁃wide DNA methylation profiles in the cortex tissues using methylat⁃ed DNA immunoprecipitation(MeDIP)combined with high⁃throughput sequencing. Results The analysis revealed 2 346 CpG sites existed only in AD mice,representing 485 unique genes as potentially associated with AD and these methylated DNA fragments distributed in different chromo⁃somes. Some hyper⁃methylated genes displayed familial aggregation. Conclusion There is significant difference in DNA methylation sites between APP/PS1 transgenic AD mice and corresponding wild mice,suggesting that DNA methylation may be involved in onset and development of AD.关键词
阿尔茨海默病/基因组/DNA甲基化/APP/PS1转基因鼠Key words
Alzheimer′s disease/genome-wide/DNA methylation/APP/PS1 transgenic mice分类
医药卫生引用本文复制引用
丛琳,张楠楠,佡剑非,任艳..甲基化芯片检测APP/PS1双转基因小鼠基因组DNA甲基化分布[J].中国医科大学学报,2015,(2):160-163,188,5.基金项目
中国医科大学附属盛京医院院内项目 （）
