中国医科大学学报Issue(3)：199-202,4.
Eaf2基因调控microRNA抑制白内障发生的机制研究
Mechanism of Eaf2 Gene Regulating microRNA in Inhibiting the Genesis of Cataract
摘要
Abstract
Objective To explore the expression of microRNA in Eaf2 knockout mice and the effect of Eaf2 on the apoptosis of human lens epithe⁃lial cells and the expression of microRNA in lens. Methods pEGFP⁃C1⁃Eaf2 was transfected into SRA01/04 cells using Lipofectamine 2000 to over express Eaf2 gene,and then the flow cytometry was used to detect cell apoptosis rate. And real time q⁃PCR was used to measure the expression of microRNA both in human lens epithelial cells and Eaf2 knockout mice. Results Compared with controls,the apoptosis rate of cells transfected with pEGFP⁃C1⁃Eaf2 was reduced,the expression of miR⁃125b and let⁃7a was significantly increased and miR⁃204 was decreased in cells transfect⁃ed with pEGFP⁃C1⁃Eaf2. Compared with controls,the expression of miR⁃125b and let⁃7a was lower and miR⁃204 was higher in Eaf2 knockout mice. Each result was statistically significant(P<0.01). Conclusion Eaf2 might inhibit apoptosis of human lens epithelial cells via regulating the expression of microRNA. Eaf2 may have a protective effect for the lens in the genesis of cataract.关键词
Eaf2/microRNA/基因调控/白内障Key words
Eaf2/microRNA/gene regulation/cataract分类
医药卫生引用本文复制引用
秦宇,赵江月,罗文婷,李晶,吴欣蔚,刘佳,阎启昌,张劲松..Eaf2基因调控microRNA抑制白内障发生的机制研究[J].中国医科大学学报,2015,(3):199-202,4.基金项目
国家自然科学基金（81270988）；辽宁省教育厅科学技术研究一般项目（L2014305）；中国医科大学附属第四医院青年创新发展基金项目 （）
