中国医科大学学报Issue(6)：562-564,572,4.
髓样分化因子88抑制剂ST2825对重组耻垢分枝杆菌感染THP-1细胞自噬的影响
The Effect of Myeloid Differentiation Factor 88 Inhibitor ST2825 on the Autophagy of THP-1 Cells Infected with Recombinant Mycobacteriumsmegmatis
胡少婷 1李升锦 1黄秦1
作者信息
- 1. 重庆医科大学附属第二医院呼吸内科，重庆 400010
- 折叠
摘要
Abstract
Objective To investigate the effect of myeloid differentiation factor 88 inhibitor ST2825 on the autophagy of THP⁃1 cells infected by re⁃combinant mycobacterium smegmatis. Methods The myeloid differentiation factor 88 inhibitor ST2825 was applied on the THP⁃1 cells infected by recombinant mycobacterium smegmatis,and three groups were defined:the test group with ST2825 treatment,the control group without ST2825 treatment,and the blank group. Autophagosomes were observed under the fluorescence microscope,and the mRNA expression of Beclin⁃1 gene and Bcl⁃2gene was analyzed by RT⁃PCR. Results Compared with the control group,the number of autophagy fluorescent dots in the test group was ob⁃viously reduced(P<0. 05),and the expression levels of Beclin 1 gene and Bcl⁃2 gene were declined as indicated by the RT⁃PCR detection. Con⁃clusion The myeloid differentiation factor 88 inhibitor ST2825 might inhibit the autophagy of THP⁃1 cells through interfering the separation of Be⁃clin⁃1 and Bcl⁃2.关键词
髓样分化因子88/ST2825/重组耻垢分枝杆菌Key words
myeloid differentiation factor 88/ST2825/recombinant mycobacterium smegmatis分类
医药卫生引用本文复制引用
胡少婷,李升锦,黄秦..髓样分化因子88抑制剂ST2825对重组耻垢分枝杆菌感染THP-1细胞自噬的影响[J].中国医科大学学报,2015,(6):562-564,572,4.
