中国药理学通报Issue(5)：646-651,6.DOI:10.3969/j.issn.1001-1978.2014.05.013
银杏内酯B对内皮细胞连接蛋白的影响及其分子机制研究
Effect of ginkgolide B on junctional proteins in oxidized LDL-stimulated human umbilical vein endothelial cells
摘要
Abstract
Aim To investigate the effect of ginkgolide B on junctional proteins in ox-LDL-stimulated human umbilical vein endothelial cells ( HUVECs) . Methods After incubation with ginkgolide B ( 0 . 2 ,0 . 4 ,0 . 6 g · L-1 ) for 1 h, HUVECs were treated with ox-LDL (0. 1 g·L-1 ) for 4 h. The expressions of JAM-A and Cx43 were analyzed with Western blot and immunofluo-rescence. The effect of ginkgolide B on vascular per-meability was analyzed by Transwell experiments. Re-sults JAM-A and Cx43 expressions increased by 22%and 24% in ox-LDL-treated HUVECs, respectively. Whereas ginkgolide B significantly decreased JAM-A and Cx43 expressions. LY294002, a specific inhibitor of PI3K, suppressed JAM-A and Cx43 expressions in ox-LDL-stimulated cells. Ginkgolide B potently re-duced monocyte migration in ox-LDL-treated cells. Conclusion Ginkgolide B significantly suppresses JAM-A and Cx43 expressions, and reduces monocyte migration in ox-LDL-stimulated cells. This demon-strates that ginkgolide B can improve vascular permea-bility. The mechanism might be associated with the in-hibition of PI3K/Akt signaling pathway.关键词
银杏内酯B/人脐静脉内皮细胞/JAM-A/Cx43/血管渗透性/炎症Key words
ginkgolide B/human umbilical vein en-dothelial cells/JAM-A/Cx43/vascular permeability/inflammation分类
医药卫生引用本文复制引用
刘雪青,陈北冬,鲍利,吴伟,孙文佳,齐若梅..银杏内酯B对内皮细胞连接蛋白的影响及其分子机制研究[J].中国药理学通报,2014,(5):646-651,6.基金项目
国家自然科学基金资助项目(No 81070231,81270379) （No 81070231,81270379）
