中国药理学通报Issue(5)：657-661,5.DOI:10.3969/j.issn.1001-1978.2014.05.015
没食子酸诱导人肝癌细胞SMMC-7721凋亡机制的探讨
Study on gallic acid induced human hepatoma SMMC-7721 cells apoptosis and its mechanism
摘要
Abstract
Aim To investigate the proliferative effect and the apoptosis of human hepatoma SMMC-7721 cells induced by gallic acid ( GA ) , and its underlying mechanism. Methods SMMC-7721 cells were cul-tured in vitro. MTT assay was used to observe the pro-liferation of SMMC-7721 cells induced on GA 24 , 48 , 72 h. The morphological and ultra structural changes of the SMMC-7721 cells were observed by inverted micro-scope and transmission electron microscope respective-ly. Annexin V-FITC/PI staining was used to quantify the percentages of apoptosis in the total cell popula-tion. The expression of p53 mRNA was investigated by RT-PCR. Western blot was used to determine the pro-tein expression of p53. Results GA(6. 25~50 μmol ·L-1 ) markedly inhibited the activity of proliferation and induced apoptosis of SMMC-7721 cells after 48 h in a dose-dependent manner. GA significantly induced cell nuclear condensation and fragmentation. RT-PCR and Western blot results showed that GA could improve the expression of p53 mRNA and protein. Conclusion GA can inhibit the proliferation of human hepatoma SMMC-7721 cells and induce cells apoptosis. The mechanism may be associated with improving tumor suppressor gene p53 expression.关键词
没食子酸/肝癌/SMMC-7721细胞/增殖/细胞凋亡/抑癌基因p53Key words
gallic acid/hepatoma/SMMC-7721 cells/proliferation/cell apoptosis/tumor suppressor gene p53分类
医药卫生引用本文复制引用
李沐涵,王明艳,赵凤鸣,陈海彬,周红光,赵青春,李文婷,吴勉华..没食子酸诱导人肝癌细胞SMMC-7721凋亡机制的探讨[J].中国药理学通报,2014,(5):657-661,5.基金项目
国家重点基础研究发展计划(973计划)中医理论专项课题(No 2006CB504807) （973计划）
国家自然科学基金资助项目(No 30973742,81273717,81102563) （No 30973742,81273717,81102563）
教育部博导课题(No 20113237110001) （No 20113237110001）
