中国药理学通报Issue(5)：725-731,7.DOI:10.3969/j.issn.1001-1978.2014.05.030
基于氨基酸代谢的抗脑缺血益气解毒配伍中药协同作用研究
Synergistic and protective effects of various combination of major components of YiQiJieDu (YQJD) on focal cerebral ischemia injury based on amino acid metabonomics
摘要
Abstract
Aim To elucidate the therapeutic effect of ginsenosides, berberine and jasminoidin after given a-lone or treatment with combination on the focal cerebral ischemia rats and study the compatibility mechanism. Methods We determined 12 endogenous amino acids in serum of rats after cerebral ischemia over 12 hours with RRLC-QQQ to evaluate the integrated role of YQJD at the dosage of 25 mg·kg-1 and 5 mg·kg-1 . Generally accepted methods were used, including be-havior test, One-Way AVONA, PLS-DA, as well as PCA to evaluate the injury induced by focal cerebral is-chemia. Results The score of neurological deficits and the level of five amino acids, namely Glu, Asp, Met, Hcy, Phe in the combination of ginsenosides, berberine and jasminoidin group in the dosage of 25 mg ·kg-1 and 5 mg·kg-1 significantly decreased (P<0. 05, P<0. 01) compared to those of model group. For another, the largest contribution group in the three principal components of PC1 , PC3 , PC4 at the dosage of 25 mg/kg and the six principal components PC1 ~PC5, PC7 in 5 mg·kg-1 was the combination of gin-senosides, berberine, jasminoidin group. Conclusions The results suggest that the efficacy of the combina-tion of ginsenosides, berberine and jasminoidin is su-perior to the combination of two or any single compo-nent, which can significantly improve the metabolic disorder of the endogenous amino acid after cerebral is-chemia. And it could be speculated that ginsenosides may play a more important role than berberine and jas-minoidin in regulating the level of amino acid metabo-lism.关键词
脑缺血/益气解毒方/氨基酸代谢/神经行为学评分/主成分分析/协同作用Key words
cerebral ischemia/YiQiJieDu/amino acid metabolism/neurological deficits score/principal com-ponent analysis/synergism分类
医药卫生引用本文复制引用
王军玲,杨阳,高健,方清影,李德凤,吴传鸿,黄志英,杨更亮,李韶菁..基于氨基酸代谢的抗脑缺血益气解毒配伍中药协同作用研究[J].中国药理学通报,2014,(5):725-731,7.基金项目
国家自然科学基金资助项目(No 81274133,81303261) （No 81274133,81303261）
国家科技部“重大新药创制”科技重大专项项目( No 2012ZX09103-201-055) （ No 2012ZX09103-201-055）
