中国药理学通报Issue(6)：787-790,791,5.DOI:10.3969/j.issn.1001-1978.2014.06.011
成人骨髓间充质干细胞体外向视网膜细胞的诱导分化
Human bone marrow-derived mesenchymal stem cells differentiate into retinal cells in vitro
摘要
Abstract
Aim To study the differentiation of human bone marrow-derived mesenchymal stem cells ( HM-SCs) into retinal cells in vitro. Methods HMSCs were isolated from human bone marrow after Ficoll den-sity gradient centrifugation. The adherent cells after at least 5 passages were used for study. Immunopheno-type of the cells was analysed by flow cytometer, and cellular differentiation was identified by immunofluores-cence labeling technique. Results The target cells derived from human bone marrow adhered to the plate with fibroblastic-like morphology, whose surface mark-ers were similar to mesenchymal stem cells. Major cells <br> were positive for CD90 , CD44 , CD147 , while they were all negative for CD34, CD45, HLA-DR. In the differentiation study, HMSCs cultured in induced me-dium can differentiate into nestin ( neural stem cell ) -positive cell, GFAP ( glial fibrillary acidic protein ) -positive glial cells and retina-specific neurons express-ing Rhodopsin with CD90 ( mesenchymal stem cells )-negative. Conclusion HMSCs have the ability to dif-ferentiate into retinal neural cells in vitro.关键词
骨髓/间充质干细胞/视网膜/体外诱导分化/免疫荧光染色/RhodopsinKey words
bone marrow/mesenchymal stem cells/retina/cellular differentiation in vitro/immunofluores-ence staining/Rhodopsin分类
医药卫生引用本文复制引用
俞海燕,吴文涛,王薇,张纯..成人骨髓间充质干细胞体外向视网膜细胞的诱导分化[J].中国药理学通报,2014,(6):787-790,791,5.基金项目
国家自然科学基金资助项目( No 30170999) （ No 30170999）
北京市科委资助项目(No H020220010390) （No H020220010390）
