中国药理学通报Issue(6)：807-811,5.DOI:10.3969/j.issn.1001-1978.2014.06.015
葫芦素E抑制HeLa细胞mTORC1的活性并诱导细胞自噬
Cucurbitacin E induces autophagy in HeLa cells by inhibiting mTORC1 activity
摘要
Abstract
Aim To study the mechanism of cucurb-itacin E ( CuE )-induced autophagy in HeLa cells. Methods Improved MTT assay was adopted to meas-ure the effect of CuE on cell proliferation. Western blot was used to determine the phosphorylation levels of downstream signaling proteins of mTORC1 and the ex-pression of autophagy associated proteins. ResultsCuE inhibited the proliferation of HeLa cells in a dose-dependent manner, and the 24-h IC50 of CuE was 4. 01μmol· L-1 . CuE significantly inhibited the phospho-rylation of p70 S6 K in a time-and dose-dependent man-ner as evidenced by decreased phosphorylation levels of <br> the mTORC1 substrate. Meanwhile, the expression of LC3-II, a marker for autophagosome formation, was elevated by CuE treatment, and was further increased in the presence of chloroquine. Furthermore, CuE re-duced the levels of p62/SQSTM1 . These results indi-cated that CuE induced autophagy in HeLa cells. The decreased levels of phosphorylated ULK1 S757 were posi-tively correlated with autophagy induction in HeLa cells. Conclusion CuE is likely to induce autophagy through inhibiting mTORC1 activity.关键词
葫芦素E/mTORC1/LC3-Ⅱ/p70S6K/HeLa细胞/细胞自噬Key words
cucurbitacin E/mTORC1/LC3-II/p70 S6 K/HeLa cells/autophagy分类
医药卫生引用本文复制引用
张晓钰,徐丽慧,赵高翔,潘浩,周单,欧阳东云,何贤辉..葫芦素E抑制HeLa细胞mTORC1的活性并诱导细胞自噬[J].中国药理学通报,2014,(6):807-811,5.基金项目
国家自然科学基金资助项目(No 81173604) （No 81173604）
中央高校基本科研业务费专项资金(No 21609403) （No 21609403）
