中国药理学通报Issue(6)：821-824,825,5.DOI:10.3969/j.issn.1001-1978.2014.06.018
黄芩苷对皮肤鳞癌细胞生长和迁移能力的影响
Effect of baicalin on proliferation and migration activity in human skin SCC cells
摘要
Abstract
Aim To investigate the effect of baicalin on cell proliferation and cell migration in human skin SCC A431 cell line. Methods The A431 cells were incu- <br> bated with 50 mg·L-1 baicalin. The protein level of cofilin-1 was assayed by Western blot. Cofilin-1 specific siRNA fragment was designed , synthesized and trans- <br> fected into A431 cells. The proliferative activity and migration ability of cells were assessed by CCK8 assay and scratch wound healing assay separately. ResultsWestern blot results showed that baicalin treatment in-hibited the cofilin-1 protein expression to 49.3% com-pared with the control group. Single baicalin treatment and cofilin-1 silencing could drease the A431 cell growth and migration. And cofilin-1 silencing signifi- <br> cantly enhanced the efficacy of baicalin. Conclusions Baicalin could significantly inhibit the tumor cell's growth and migration in the A431 cell line. And cofi-lin-1 might become the potential target gene to enhance the effect of anticancer drugs.关键词
黄芩苷/cofilin-1/皮肤鳞癌A431 细胞/siRNA/细胞增殖/细胞迁移Key words
baicalin/cofilin-1/skin squamous cancer A431 cell/siRNA/cell proliferation/cell migration分类
医药卫生引用本文复制引用
杨子良,骆丹,林秉奖,钱齐宏,余秀琴,王淼淼,闵玮..黄芩苷对皮肤鳞癌细胞生长和迁移能力的影响[J].中国药理学通报,2014,(6):821-824,825,5.基金项目
国家自然科学基金资助项目( No 30876232) （ No 30876232）
江苏省自然科学基金资助项目( No BK2012170) （ No BK2012170）
江苏省卫生厅中医药科技项目(No LZ11095) （No LZ11095）
