中国药理学通报Issue(7)：942-946,947,6.DOI:10.3969/j.issn.1001-1978.2014.07.013
姜黄素对人循环纤维细胞增殖及CO LⅠ表达影响的研究
Effects of curcumin on proliferation and COL I expression of human circulating fibrocytes
摘要
Abstract
Aim To isolate and characterize the human circulating fibrocytes from human peripheral blood and explore the effects of curcumin on human circulating fi-brocytes.Methods The cells were isolated and puri-fied by density gradient centrifugation,and identified by flow cytometry and immunocytochemistry .Then , CCK-8 and flow cytometry were used to study the effect of curcumin on the proliferation as well as COL I ex-pression of human circulating fibrocytes,respectively. Results After being isolated the cells expressed CD34,CD45 and COLⅠ,among which 79.7% were both CD45 and collagen I positive,typical of human circulating fibrocytes.Curcumin could exert regulatory effects on proliferation of human circulating fibrocytes. Exposure of the cells to curcumin for as short as 24 hours promoted their growth,while prolonged treatment (72 h ) significantly inhibited cell propagation and downregulated the COLⅠ levels,best manifested at a concentration as high as 20 μmol · L-1 .Conclusion The proliferation of cells and COLⅠexpressions can be effectively inhibited by curcumin with the prolonged action period and high concentrations.关键词
人循环纤维细胞/姜黄素/COLⅠ/CD34/CD45/鉴定/增殖/表达Key words
human circulating fibrocytes/curcumin/COLⅠ/CD34/CD45/identification/proliferation/ex-pression分类
医药卫生引用本文复制引用
富徐燕,赵丕文,李亚东,唐雨晴,牛建昭,王鸿捷,李彧..姜黄素对人循环纤维细胞增殖及CO LⅠ表达影响的研究[J].中国药理学通报,2014,(7):942-946,947,6.基金项目
国家自然科学基金资助项目（No 81173642）；教育部国家外专局创新引智基地项目 （）
