中国药理学通报Issue(7)：1000-1005,1006,7.DOI:10.3969/j.issn.1001-1978.2014.07.025
硫化氢对离体大鼠急性缺血心肌心功能的影响
Effects of hydrogen sulfide on cardiac function induced by acute myocardial ischemia in isolated hearts of rats
摘要
Abstract
Aims To observe the changes of endoge-nous hydrogen sulfide/cystathionine-γ-lyase (H2 S/CSE)system and to study the effects of H2 S on cardiac function,H2 S/CSE and myocardial infarct volumes in acute myocardial ischemic injury in isolated hearts of rats.Methods The myocardial ischemic injury model was established by the ligation of coronary artery.The hemodynamic parameters,such as the left ventricular developed pressure (LVDP),±dp/dtmax and coronary arterial flow(CF),were respectively recorded to evalu-ate the cardiac function.The content of H2 S and the activity of CSE in cardiac tissue were detected respec-tively at each time point after ischemia.Infarct vol-umes in isolated rat hearts were determined by dual staining with Evans-blue and TTC.Results (1 )Com-pared with those of the sham group,LVDP,±dp/dtmax and CF were significantly decreased at 30 min,1,2,3, 4 h after ischemia(P<0.01),there were no statistical-ly significant differences in the content of H2 S and the activity of CSE in cardiac tissue at 3 0 min after ische-mia.But during the periods from 1 h to 4h after ische-mia,the content of H2 S and the activity of CSE in car-diac tissue were significantly decreased,the infarct volumes were greatly increased compared with those of the sham control group (P<0.05 or P<0.01 ).(2) Compared with those of the ischemia 4h group,LVDP, ±dp/dtmax and CF were significantly increased in the NaHS low,middle and high dose groups (P<0.05 or P<0.01),the content of H2S and the activity of CSE in cardiac tissue were significantly increased in the NaHS low,middle and high dose groups(P<0.05 or P<0.01 ),and the infarct volumes were significantly decreased in NaHS middle and high dose groups(P<0.01 ).Conclusion H2S and CSE are involved in myocardial ischemic injury in isolated hearts of rats. Administration of NaHS could enhance the activity of CSE,increase the content of H2 S,and reduce infarct volumes.It could be suggested that H2 S has cardiopro-tective effects on acute myocardial ischemic injury.关键词
硫化氢/急性心肌缺血/Langendorff/大鼠/心功能/胱硫醚-γ-裂解酶Key words
hydrogen sulfide/acute myocardial ische-mia/Langendorff/rat/cardiac function/cystathionine-γ-lyase分类
医药卫生引用本文复制引用
谢英花,马燕山,张楠,张建新..硫化氢对离体大鼠急性缺血心肌心功能的影响[J].中国药理学通报,2014,(7):1000-1005,1006,7.基金项目
河北省自然科学基金资助项目（No C2009001458）；河北省应用基础研究计划重点基础研究项目 （）
