中国药理学通报Issue(8)：1101-1106,6.DOI:10.3969/j.issn.1001-1978.2014.08.013
甲基苯丙胺所致的大鼠神经毒性损伤及nNOS抑制剂的保护作用
Neurotoxicity induced by methamphetamine and the protective role of nNOS inhibitor in rats
摘要
Abstract
Aim Tostudytheprotectiveeffectsofin-hibition of neuronal nitric oxide synthase ( nNOS ) in methamphetamine ( METH ) induced neurotoxicity via oxidativestressinjuryinrats.Methods Ratmodelsof acute METH poisoning with/without 7 nitroindazole (7-NI) pretreatment were built to evaluate the protec-tive effects on the changes of ethology, nNOS, nitro-proteins, dopamine ( DA ) and apoptosis in rat stria-tum.Results ThenNOSexpression,nitroproteinex-pression and apoptosis increased significantly in the METH group( P<0. 05 ) and 7-NI pretreatment shows a dramatically protective effect against the changes ( P<0. 05 ) . DA concentration decreased significantly in the METH group(P<0. 05) and 7-NI pretreatment re-stored the depleted DA to normal level ( P >0. 05 ) . Stereotyped behavior increased significantly in the METH group and the 7-NI combined group compared with the 7-NI group and the control group(P<0. 01). Conclusions 7-NIshowssignificantprotectiveeffects against the alterations of DA level, nNOS expression, nitroprotein expression and apoptosis in rat striatum caused by acute METH poisoning. However, there is no obvious protective effect on METH-induced stereo-typed behavior.关键词
甲基苯丙胺/神经毒性/神经元型一氧化氮合酶/7-硝基吲唑/氧化应激/凋亡Key words
methamphetamine/neurotoxicity/nNOS/7-nitroindazole/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
徐静涛,张付,杨幸怡,谢卫兵,李丽增,刘超,王慧君..甲基苯丙胺所致的大鼠神经毒性损伤及nNOS抑制剂的保护作用[J].中国药理学通报,2014,(8):1101-1106,6.基金项目
国家自然科学基金资助项目(No 81273345) （No 81273345）
