中国药理学通报Issue(8)：1165-1170,6.DOI:10.3969/j.issn.1001-1978.2014.08.026
ASIC1 a对大鼠关节软骨细胞基质代谢及MAPK信号通路表达的影响
Role of acid-sensing ion channel 1 a in matrix turnover and MAPK expression of articular chondrocytes of rats
摘要
Abstract
Aim TostudytheroleofASIC1aonthe matrix turnover and MAPK expression of the rat articu-lar chondrocytes with extracellular acidosis. Methods ArticularchondrocyteswereisolatedfromSprague-Dawley rats, and their phenotype was determined by toluidine blue and immunocytochemical staining. The GAG content of cell culture supernatant was deter-mined by dimethyl-methylene blue spectrophotometric assay, while Hyp content by chloramine T assay. ELISA assay was used to measure MMP-2 , TIMP-2 content. Furthermore, the ERK1/2, p38 MAPK phos-phorylation protein expression levels were tested by Westernblotassay.Results ASIC1acontributedto the effect of GAG, Hyp and TIMP-2 levels reduction induced by extracellular acidification, while the effect of MMP-2 was weaker. Moreover, ASIC1a could in-crease the ERK1/2 , p38 MAPK phosphorylation pro-teinexpressionlevels.Conclusion ASIC1acould regulate rat articular chondrocytes matrix turnover via ERK1/2 and p38 MAPK signaling pathway, and there-by inhibit the rat articular cartilage damage induced by acidosis.关键词
酸敏感离子通道1a/关节软骨细胞/基质代谢/丝裂原激活蛋白激酶/羟脯氨酸/糖胺聚糖Key words
acid sensing ion channel 1 a/articular chondrocytes/matrix metabolism/mitogen-activated protein kinase/hydroxyproline/glycosaminoglycan分类
医药卫生引用本文复制引用
张礼菊,胡伟,唐杰,吴繁荣,葛金芳,陈飞虎,吴建贤..ASIC1 a对大鼠关节软骨细胞基质代谢及MAPK信号通路表达的影响[J].中国药理学通报,2014,(8):1165-1170,6.基金项目
国家自然科学基金资助项目( No 81271949) （ No 81271949）
安徽省自然科学基金面上项目(No 1408085MH151) （No 1408085MH151）
教育部高等学校博士学科点专项科研基金项目(No 20133420120002) （No 20133420120002）
