中国药理学通报Issue(9)：1229-1234,1325,7.DOI:10.3969/j.issn.1001-1978.2014.09.011
镰刀菌色素抑制MCF-7乳腺癌细胞增殖的机制
Inhibitory effect of pigment produced by Fusarium sp JN158 on MCF-7 cell proliferation
摘要
Abstract
Aim To explore new ways for developing anticancer drugs by the separation of pigment from Fu-sarium species JN158 ( Fusarium sp JN158 ) , the iden-tification of its structure, the screening of anticancer components and the study of its partial mechanism. Methods Pigment separation was done by HPLC, structural analysis by UV, IR, NMR, the screening of anticancer activity by MTT. Western blot was used to analyze the protein expression of CyclinD1, NF-κB, VEGF in tumor cells. Results The results showed that the pigment from Fusarium produced a total of six different peaks, of which peak Ⅵ was the anthocya-nins. Its molecular weight is about 382, molecular for-mula is C17 H18 O10 . According to investigation, this pig-ment was probably a new compound, which could in-hibit the proliferation of MCF-7 cells markedly ( IC50:0.011mmol·L-1 ,P<0.05;the control medicine ube-nimex IC50:10 mmol · L-1 ) in a concentration-de-pendent manner, and had no effect on human umbilical cord intravenous endotheliocyte ( HUVEC ) . The influ-ence on the gene expression of CyclinD1, NF-κB, VEGF in MCF-7 cells varied with the concentration of this compound. The Western blot results showed that VI pigment compound inhibited CyclinD1, NF-κB, VEGF gene expression (P<0.05 or 0. 01),compared with the control group. Conclusion The Ⅵ pigment compound from Fusarium sp JN158 could inhibit MCF-7 proliferation by inhibiting CyclinD1, NF-κB, VEGF gene expression. The compound may be a promising compound against breast cancer.关键词
镰刀菌色素/分离/结构鉴定/乳腺癌细胞/细胞增殖/蛋白表达Key words
Fusarium pigments/pigment separation/structural identification/breast cancer cell/cell prolif-eration/protein expression分类
医药卫生引用本文复制引用
郑里翔,蔡宇杰,孟宪明,徐敏娟,李昌伟,王巧凤,王月,廖祥儒..镰刀菌色素抑制MCF-7乳腺癌细胞增殖的机制[J].中国药理学通报,2014,(9):1229-1234,1325,7.基金项目
国家自然科学基金资助项目( No 81160531) （ No 81160531）
国家级大学生创新创业训练计划项目(No 201210412) （No 201210412）
江苏省科技创新与成果转化(重大科技支撑与自主创新)专项引导资金(No BY2010117) （重大科技支撑与自主创新）
