中国药理学通报Issue(9)：1276-1280,5.DOI:10.3969/j.issn.1001-1978.2014.09.020
下丘脑室旁核ATP敏感性钾离子通道参与大鼠炎性痛调节的作用研究
Contributions of K ATP within the paraventricular nucleus in the modulation of inflammatory pain in rats
摘要
Abstract
Aim To explore the role of KATP in the pa-raventricular nucleus in inflammatory pain. MethodsMale Sprague-Dawley rats,250~280 g, were randomly assigned into 5 groups ( each, n =6 ): Normal group, Saline group ( for control, subcutaneous injection of 100 μl of saline into the plantar surface of the left hindpaw) , CFA group ( subcutaneous injection of 100μl of complete freund's adjuvant into the plantar sur-face of the left hindpaw) , Vehicle group ( treated with dimethylsulfoxide), KATP selective agonist group(trea-ted with diaoxide) . Rats in each group were tested for TWL with radiant heat apparatus. Immunofluorescent technique was used to label KATP in PVN and c-Fos in lumber spinal cord. Three days after injected with CFA, a selective KATP agonist, diaoxide, was injected into one side of PVN to test its effect on inflammatory pain and c-Fos expression in lumber spinal cord. Re-sults ① Compared with pre-operation and saline group, rats showed significantly lower TWL on day 1, 3, 7 after injection of CFA;the numbers of KATP posi-tive cells were significantly lower; the numbers of c-Fos positive cells were significantly higher. ② Com-pared with those of vehicle group, TWL and the num-ber of c-Fos in lumber spinal cord were both signifi-cantly lower after injection of diaoxide into one side of PVN. Conclusion KATP in PVN is related to inflam-matory pain.关键词
ATP敏感性钾离子通道/下丘脑/室旁核/完全弗氏佐剂/炎性痛/二氮嗪Key words
ATP-sensitive potassium channel/hypo-thalamic/paraventricular nucleus/complete freund's adjuvant/inflammatory pain/diaoxide分类
医药卫生引用本文复制引用
张秀丽,闫巍巍,刘东志,张励才..下丘脑室旁核ATP敏感性钾离子通道参与大鼠炎性痛调节的作用研究[J].中国药理学通报,2014,(9):1276-1280,5.基金项目
国家自然科学基金资助项目( No 81371243) （ No 81371243）
江苏省自然科学基金资助项目(No BK2012580) （No BK2012580）
