中国药理学通报Issue(10)：1367-1371,1372,6.DOI:10.3969/j.issn.1001-1978.2014.10.009
牛磺酸镁对缺氧/复氧致大鼠心肌细胞钙离子通道异常的影响
Effects of taurine-magnesium coordination compound on abnormal calcium current induced by hypoxia-reoxygenation in rat ventricular cardiomyocytes
摘要
Abstract
Aim To investigate the effects of TMCC on abnormal L-type calcium current (ICa,L) in rat ventric-ular cardiomyocytes during hypoxia-reoxygenation to find out the mechanism of antiarrhythmic effect. Methods Whole-cell patch clamp was used to record ICa,L in the ventricular cardiomyocytes during hypoxia-reoxygenation in rat under amiodarone and different concentrations of TMCC. Results In hypoxia-reoxy-genation model, peak ICa,L increased from ( 3. 35 ± 0. 50 ) pA/pF to ( 5. 69 ± 0. 25 ) pA/pF ( n =6 , P <0. 01 ) , which was restored to ( 5. 28 ± 0. 18 ) pA/pF (n=6, P>0. 05),(4. 41 ± 0. 22) pA/pF, (3. 82 ± 0. 21)pA/pF(n=6, P<0. 01) by TMCC(100, 200, 400 μmol·L-1 ) and amidodarone 24. 24 μmol·L-1 restored peak ICa,L to(3. 66 ± 0. 27)pA/pF (n=6,P<0. 01 ) . Compared to control group, hypoxia-reoxy-genation turned ICa,L steady-state activation curves to left and inactivation curves to right, which quickened activation and slowed inactivation, TMCC ( 200, 400μmol · L-1 ) and amiodarone could restore the left shift activation curves and right shift inactivation curves. Conclusion TMCC can concentration-de-pendently restore the increase of calcium current due to hypoxia-reoxygenation by promoting inactivation process and inhibiting activation process, and the effect is equal to that of amiodarone. TMCC blocks ICa,L of the ventricular cardiomyocytes, which may be one of its antiarrhythmic mechanisms.关键词
牛磺酸镁/心律失常/心室肌细胞/钙离子通道/全细胞膜片钳/胺碘酮Key words
taurine-magnesium coordination com-pound/arrhythmia/ventricular cardimyocytes/calcium ion channel/whole-cell patch clamp technique/amiod-arone分类
医药卫生引用本文复制引用
张铭慧,尹永强,康毅,娄建石..牛磺酸镁对缺氧/复氧致大鼠心肌细胞钙离子通道异常的影响[J].中国药理学通报,2014,(10):1367-1371,1372,6.基金项目
国家自然科学基金资助项目(No 30672458) （No 30672458）
