中国药理学通报Issue(10)：1372-1376,5.DOI:10.3969/j.issn.1001-1978.2014.10.010
胱硫醚-β-合酶/硫化氢系统在鱼藤酮诱导PC12细胞损伤中的变化
Changes of endogenous cystathionine-β-synthase/hydrogen sulfide system in PC12 cells injury induced by rotenone
摘要
Abstract
Aim To research dynamically the changes of endogenous cystathionine- β-synthase/hydrogen sul-fide system in PC12 cells injury induced by rotenone. Methods Rotenone-induced injury in PC12 cells ( characteristic of dopaminergic neurons) was used as a PD cell model. The expression of CBS was evaluated by Western blot. Intracellular CBS activity and H2 S production were detected by Methylene blue spectro-phot-ometric method. The viability of PC12 cells was measured by CCK-8 assay. GSH detection kit was used to detect the intracellular GSH content. Results In the groups of 6 and 12 hours, the expression and activ-ity of CBS were elevated, and the production of H2 S was increased. In the groups of 24 and 48 hours, CBS expression and activity were significantly decreased, and the amount of H2 S was significantly reduced. Ap-plication of 1. 5 μmol·L-1 rotenone for different time (6-48h) could decrease the cell viability and intra-cellular GSH contents in a time-dependent manner. Conclusions The expression and activity of endoge-nous CBS, stimulated by rotenone, are elevated firstly and then decreased. The generation of H2 S, stimulated by rotenone, is increased and then reduced significant-ly, which may be related to PC12 cells against oxida-tive stress damage induced by rotenone.关键词
鱼藤酮/帕金森病/PC12细胞/硫化氢/胱硫醚-β-合酶/氧化应激Key words
rotenone/Parkinson′s disease/PC12 cells/hydrogen sulfide/cystathionine-β-synthase/oxi-dative stress分类
医药卫生引用本文复制引用
许岩,马娜,邓树泳,王金全,冯鉴强,孟金兰..胱硫醚-β-合酶/硫化氢系统在鱼藤酮诱导PC12细胞损伤中的变化[J].中国药理学通报,2014,(10):1372-1376,5.基金项目
广东省高校优秀青年创新人才培养计划资助项目( No 2012LYM_0083) （ No 2012LYM_0083）
广东省医学科研基金资助项目( No A2011295) （ No A2011295）
广东省高等学校大学生创新创业训练计划项目(No 1057313016) （No 1057313016）
2013年广东药学院大学生创新性实验计划资助项目 （）
