中国药理学通报Issue(10)：1388-1391,1392,5.DOI:10.3969/j.issn.1001-1978.2014.10.013
PLCε对人骨肉瘤细胞迁移侵袭影响的实验研究
Experimental study of PLCεon migration and invasion of human osteosarcoma cancer cells
摘要
Abstract
Aim To investigate the effects of PLCε on the invasion and migration of human osteosarcoma cancer cells U2OS. Methods RNA interference ( RNAi) was used to inhibit PLCεexpression, and the proliferation of cancer cells was measured by CCK-8 assay. The migration of the cells was measured by scratch wound healing assay and migration chamber as-say. Gelatin zymography was performed to measure the MMP2 activities in U2OS cells. Results PLCε ex-pression was suppressed by siRNA. CCK-8 assay showed that PLCε had no effect on the proliferation of cancer cells. PLCε knockdown inhibited cell invasion and activities of MMP2 . Conclusion PLCε knock-down can inhibit the migration and invasion of human osteosarcoma cancer cells U2OS.关键词
PLCε/siRNA/增殖/迁移/侵袭/骨肉瘤Key words
PLCε/siRNA/proliferation/migration/invasion/osteosarcoma分类
医药卫生引用本文复制引用
吴金霞,曹文嘉,桑苗苗,郑骏年,裴冬生..PLCε对人骨肉瘤细胞迁移侵袭影响的实验研究[J].中国药理学通报,2014,(10):1388-1391,1392,5.基金项目
国家自然科学基金资助项目(No 81372172) （No 81372172）
教育部重点课题项目(No 212062) （No 212062）
