中国药理学通报Issue(10)：1419-1424,6.DOI:10.3969/j.issn.1001-1978.2014.10.019
EGCG通过抑制p75 NTR通路对APP/PS1小鼠学习记忆障碍的改善作用
EGCG ameliorating learning and memory impairment in APP/PS1 mice by inhibiting p75 NTR pathway
摘要
Abstract
Aim To investigate whether EGCG treat-ment ameliorates cognitive deficits in APP/PS1 trans-genic mice and, whether it has the ameliorating effect of p75 NTR signaling to neuronal apoptosis in the hippo-campus of APP/PS1 mice. Methods Morris water maze test and locomotivity test were used to predict be-havioral changes; further TUNEL staining and Fluoro-Jade B staining were applied to confirm the neuronal apoptosis and neuronal degeneration;Western blot was employed to detect protein expression levels of p75 NTR signaling in the hippocampus of APP/PS1 mice. Re-sults EGCG treatment dramatically ameliorated the cognitive impairments, and inhibited the neuronal ap-optosis in the APP/PS1 mice. Moreover, EGCG treat-ment dramatically inhibited the p75 NTR signaling by de-creasing the p75ICD expression, JNK2 phosphorylation, and cleaved-caspase 3 expression. Conclusion EGCG treatment dramatically ameliorates the cognitive impairments, and inhibits the neuronal apoptosis by in-hibiting the p75NTR signaling.关键词
EGCG/NGF/p75NTR信号通路/阿尔采末病/APP/PS1转基因小鼠/学习记忆障碍Key words
EGCG/NGF/p75NTR signaling/Alzhei-mer′s disease/APP/PS1 transgenic mice/learning and memory impairment分类
医药卫生引用本文复制引用
杨时伦,刘明妍,钟欣,杜可,姚维范,赵海山,魏敏杰..EGCG通过抑制p75 NTR通路对APP/PS1小鼠学习记忆障碍的改善作用[J].中国药理学通报,2014,(10):1419-1424,6.基金项目
国家科技部“重大新药创制”科技重大专项资助项目( No 2013ZX09103001-003) （ No 2013ZX09103001-003）
辽宁省科学技术计划项目( No 2011415052,2013225079) （ No 2011415052,2013225079）
辽宁省教育厅科学研究一般项目(No L2012279) （No L2012279）
